| 黄军凯; 张国珍*.促进草莓炭疽病菌大量产孢的方法[J].植物保护,2014,40(4):107-111. |
| 促进草莓炭疽病菌大量产孢的方法 |
| A method for inducing sporulation of Colletotrichum pathogen from strawberry |
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| DOI： |
| 中文关键词: 草莓炭疽病菌 诱导产孢 酵母提取物 涂断菌丝 |
| 英文关键词:Colletotrichum theobromicola inducing sporulation yeast extract erasing aerial mycelium |
| 基金项目:公益性行业农业科研专项201003064-4，201303025 |
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| 中文摘要: |
| 为了促进草莓炭疽病菌在人工培养基上快速、大量地产生分生孢子，本研究在PDA培养基中添加不同量的酵母提取物，并结合涂断气生菌丝的方法，测定了不同处理对草莓炭疽病菌分生孢子产生的影响。结果发现，在PDA培养基中添加0.1%酵母提取物最适合草莓炭疽病菌（Colletotrichumtheobromicola）的产孢。将培养3 d菌落上的气生菌丝涂断，2 d后可产生大量分生孢子。因此，PDA培养基中添加0.1%酵母提取物，结合菌丝涂断的方法，可显著提高草莓炭疽病菌的产孢量。该方法所用营养成分简单，用时短，操作简便。 |
| 英文摘要: |
| In order to induce Colletotrichum pathogen from strawberry to produce a large number of conidia on media in a short time, the effects of two methods, i.e., adding different contents of yeast extract (YE) to PDA medium and erasing the air mycelia on PDA, on sporulation of C. theobromicola were determined. The results indicated that PDA+0.1% YE was the optimal medium for sporulation of C. theobromicola. When the aerial mycelia growing 3 days on PDA medium were erased and continuously cultured for 2 days, the sporulation of the pathogen could be increased significantly. Therefore, using PDA added with 0.1% YE and combined with erasing aerial mycelia is a convenient method for inducing massive sporulation of C. theobromicola. |
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