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赖宝春1,胡先奇2,罗文富2.云南灯盏花根腐病的2种新病原鉴定及防治药剂的室内筛选[J].植物保护,2014,40(2):140-145.
云南灯盏花根腐病的2种新病原鉴定及防治药剂的室内筛选
Identification of the two new pathogens of root rot disease on Erigeron breviscapus and screening of fungicides
  
DOI:
中文关键词:  灯盏花  根腐病  病原鉴定  毒力测定  致病性
英文关键词:Erigeron breviscapus  root rot disease  identification of pathogen  toxicity test  pathogenicity
基金项目:
作者单位
赖宝春1,胡先奇2,罗文富2 1. 福建省漳州市农业科学研究所漳州363005 2. 云南农业大学云南省植物病理重点实验室 昆明650201 
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中文摘要:
      引起云南省灯盏花根腐病的病原为茄病镰刀菌(Fusarium solani)、尖孢镰刀菌(F.oxysporum)和半裸镰刀菌(F.semitectum)3种真菌,茄病镰刀菌和尖孢镰刀菌为灯盏花根腐病的2种新病原,其中茄病镰刀菌为主要致病菌,其分离率为42.86%,茄病镰刀菌有伤接种发病率为70%,无伤接种发病率为56.7%。采用室内生长速率法测定了50%多菌灵可湿性粉剂、70%甲基硫菌灵可湿性粉剂、64%恶霜·锰锌可湿性粉剂、10%苯醚甲环唑水分散粒剂、10亿活芽胞/g 枯草芽胞杆菌·荧光假单胞杆菌可湿性粉剂5种供试杀菌剂对灯盏花根腐病主要病原茄病镰刀菌的毒力。结果表明:5种供试药剂对茄病镰刀菌都有抑制作用。对茄病镰刀菌的有效中浓度(EC50)分别为0.000 4、0.050 7、0.053 5、0.081 3和8.624 0 mg/mL,其中50%多菌灵可湿性粉剂有效中浓度最低,相对抑制效果最好。5种药剂的毒力相关系数均在0.89以上,表明药剂质量浓度与抑制作用呈较高相关性。
英文摘要:
      The three pathogens causing root rot of Erigeron breviscapus in Yunnan Province were identified as Fusariumsolani, F.oxysporum and F.semitectum, among which F. solani and F.oxysporum were two new pathogens; the main pathogen was F.solani, and the isolation frequency was 42.86%. After artificial inoculation with or without wounds, the incidence of roots infected by F.solani was 70% and 56.7%, respectively. With mycelium growth rate test method, five fungicides to F.solani had inhibition effects, indicating that the EC 50 values of 50% carbendazim WP, 70% thiophanate methyl WP, 64% oxadixyl·mancozeb WP, 10% difenoconazole WG, and 1 billion live spores of Bacillus subtilis and Pseudomonas fluorescens WP were 0.000 4 mg/mL, 0.050 7 mg/mL, 0.053 5 mg/mL, 0.081 3 mg/mL and 8.624 0 mg/mL, respectively, suggesting that 50% carbendazim WP was the best. The correlation coefficients for the five fungicides were all above 0.89, indicating that mass concentration had high correlation with inhibition.
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