| 王祥1，李刚1，赵黎明1，刘金亮2，辛志梅3，竺晓平1*.吉林番茄黄化曲叶病毒分离物的检测及其DNA-A全基因组序列分析[J].植物保护,2014,40(2):76-80. |
| 吉林番茄黄化曲叶病毒分离物的检测及其DNA-A全基因组序列分析 |
| Molecular identification and DNA-A gene sequence analysis of TYLCV isolates from Jilin Province |
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| DOI： |
| 中文关键词: 吉林省 番茄黄化曲叶病毒 分子鉴定 双生病毒 |
| 英文关键词:Jilin Province TYLCV molecular identification geminiviruses |
| 基金项目: |
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| 摘要点击次数: 687 |
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| 中文摘要: |
| 从吉林省松原市田间表现黄化曲叶症状的番茄上分离到病毒分离物JLSY，基因组全序列测定结果表明，基因组全长2781 bp，共编码6个ORF。序列比对表明，JLSY基因组与番茄黄化曲叶病毒（TYLCV）安徽分离物（FN650807）相似性最高，为99.5%，而与其他双生病毒的序列相似性低于89%。经系统进化分析，该病毒分离物属于TYLCV以色列株系（TYLCV-IL）。这是首次在我国吉林省检测到番茄黄化曲叶病毒。 |
| 英文摘要: |
| A virus isolate JLSY was obtained from tomato plants with symptoms of leaf curl and yellowing in Songyuan, Jilin Province. Complete nucleotide sequence of the genome of JLSY was determined to be 2781 nucleotides encoding 6 ORFs. Sequence analysis showed the complete genomic sequence of JLSY has highest identity of 99.5% with tomato yellow leaf curl virus isolate of Anhui (FN650807), while less than 89% nucleotide sequence identities with other Begomoviruses. Phylogenic analysis results indicated that JLSY is an isolate of the tomato yellow leaf curl virus Israel strain (TYLCV IL). This is the first report of tomato yellow leaf curl virus in Jilin Province. |
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