| 侯吉祥，孟盼盼，马伟华，周菲，林拥军*.黑尾叶蝉精氨酸激酶和一个谷胱甘肽S转移酶基因的克隆和发育表达特性研究[J].植物保护,2014,40(1):84-89. |
| 黑尾叶蝉精氨酸激酶和一个谷胱甘肽S转移酶基因的克隆和发育表达特性研究 |
| Cloning and expression profiles of arginine kinase and GSTs of Nephotettix cincticeps |
| |
| DOI： |
| 中文关键词: 黑尾叶蝉 精氨酸激酶 谷胱甘肽-S-转移酶 |
| 英文关键词:Nephotettix cincticeps arginine kinase glutathione S transferase |
| 基金项目: |
|
| 摘要点击次数: 1349 |
| 全文下载次数: 1407 |
| 中文摘要: |
| 黑尾叶蝉（Nephotettix cincticeps）是一种能传播病毒的水稻害虫，对水稻生产有着巨大的威胁。本文在黑尾叶蝉中克隆得到了一个精氨酸激酶基因（NcAK）和一个delta类型的谷胱甘肽-S-转移酶基因（NcGST），并对两个基因进行了序列分析和表达特性检测。结果表明，NcAK包含的开放阅读框长度为1 068 bp，编码一个含356个氨基酸的蛋白。进化研究表明该蛋白序列与玻璃翅叶蝉（Homalodisca vitripennis）的精氨酸激酶的序列亲缘关系最近。Real time结果表明NcAK在黑尾叶蝉的雄成虫中表达量高于在卵、1龄若虫、5龄若虫和雌成虫中的表达量。NcGST的开放阅读框由651个碱基组成，编码一个包含217氨基酸的蛋白，进化研究表明该蛋白与美国牧草盲蝽(Lygus lineolaris)GSTs编码蛋白亲缘关系最近。表达量检测表明NcGST在5龄若虫和雌雄成虫中的表达量要高于在卵和1龄的表达量。 |
| 英文摘要: |
| The green leafhopper Nephotettix cincticeps is a rice insect that can transmit plant viruses. We cloned and analyzed an arginine kinase gene named NcAK and a delta class GSTs gene named NcGST in Nephotettix cincticeps. NcAKcontained an open reading frame (ORF) of 1 068 bp in length, and encodes a protein of 356 amino acids. Phylogenetic analysis showed that the deduced protein of NcAK was closely related to the arginine kinase of Homalodisca vitripennis and Oncometopia nigricans. The real-time PCR results indicated that the NcAK was expressed higher in male adults than in the eggs, 1st instars, 5th instars, and female adults. NcGST, 899 bp in length, contained an ORF of 651 bp and encoded a protein of 217 amino acids. Phylogenetic analysis showed that the deduced protein of NcGST was closely related to the GSTs of Lygus lineolaris. The real time PCR results indicated that NcGST was expressed higher in 5th instars, male adults and female adults than in eggs and 1st instars. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
