| 孙书娥1，李萌2,3，朱春晖2,3，谭新球2,3，刘勇1,2,3，张德咏1,2,3*.番茄斑萎病毒YN-1株系GN/GC基因的克隆与分析[J].植物保护,2014,40(1):65-69. |
| 番茄斑萎病毒YN-1株系GN/GC基因的克隆与分析 |
| Cloning and sequence analysis of the GN/GC gene of Tomato spotted wilt virusYN-1 |
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| DOI： |
| 中文关键词: 番茄斑萎病毒 GN/GC基因 克隆 分析 |
| 英文关键词:Tomato spotted wilt virus G N/G C gene clone analysis |
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| 中文摘要: |
| 作者以TSWV YN-1株系为研究材料，克隆了其G N/G C基因，测序后与GenBank中TSWV其他株系GN/GC基因进行比对，结果显示：YN-1 G N/G C基因全长3 408 bp，编码1 135个氨基酸，构建的系统发育树可分为4个群，YN-1属于群Ⅱ，与源自西班牙的SPAIN-2株系的G N/G C基因核苷酸相似性最高，达98.88%，氨基酸相似性达98.85%。YN-1株系N基因和G N/G C基因的起源不同，我们推断YN-1可能是一个发生重排（reassortment）的新株系，是不同地理起源的株系在共同传播过程中发生了重排的结果，具体原因有待进一步研究。 |
| 英文摘要: |
| In this study, we cloned the complete nucleotide sequence of GN/GC gene of TSWV YN-1 strain, and analyzed its phylogenetic relationship to other TSWV isolates from GenBank. The results showed that the complete nucleotide sequence of GN/GC gene was 3 408 nt and encoded 1 135 aa, and the phylogenetic tree of the complete nucleotide sequence of the G N/G C gene of TSWV isolated from different hosts in different geographic origins in the world, divided into four groups, were constructed, where TSWV YN-1 was in group Ⅱ, and the complete nucleotide and amino acid sequences of G N/G C exhibited 98.88% and 98.85% identity with SPAIN-2 (accession number: AY744493) isolated from tomato in Spain, respectively. Because the origins of N gene and G N/G C ene of TSWV YN-1 were different, we inferred that TSWV YN-1 might be a new strain, resulted from a reassortment event happened in co transmission between different strains of geographic origins. The mechanism needs to be studied further. |
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