| 王玉琴，杨成德*，陈秀蓉，薛莉，苏建红.甘肃省马铃薯枯萎病（Fusarium avenaceum）鉴定及其病原生物学特性[J].植物保护,2014,40(1):48-53. |
| 甘肃省马铃薯枯萎病（Fusarium avenaceum）鉴定及其病原生物学特性 |
| Identification of potato wilt caused by Fusarium avenaceum and the biological characteristics of its pathogen |
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| DOI： |
| 中文关键词: 马铃薯 枯萎病 鉴定 培养性状 |
| 英文关键词:potato Fusarium wilt identification culture characteristics |
| 基金项目: |
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| 中文摘要: |
| 对甘肃省马铃薯枯萎病菌进行了分离、鉴定及培养特性的研究。结果表明，该病原菌大型分生孢子镰刀形，无色，多细胞，大小为（12.5~30）μm×（2.5~5）μm；小型分生孢子数量较多，无色，卵圆形或椭圆形，大小为（4.70~12.94）μm×（1.76~2.35）μm；ITS序列分析表明，病原菌与Fusarium avenaceum（JN6317483）亲缘关系最近，相似性达到100%，结合形态特征鉴定该病原菌为燕麦镰刀菌(Fusarium avenaceum)。该病原菌在5~40 ℃范围内均可生长，最适温度为20 ℃；供试的碳、氮源中，果糖和亮氨酸对该菌菌丝生长有显著的促进作用（P<0.05），而显著抑制菌丝生长的是氯醛糖和碳酸铵（P<0.05）；不同类型培养基中，燕麦片培养基对菌丝生长有显著促进作用（P<0.05）。 |
| 英文摘要: |
| The pathogen causing potato wilt were isolated and identified by the rDNA ITS sequences and biological characteristics in Gansu Province. The results showed that the macroconidia were hyaline, sickle shaped, multi cellular, and the size was (12.5-30)μm × (2.5-5)μm. The microconidia were oval, and the size was (4.70-1294) μm×(1.76-2.35)μm. The rDNA ITS sequence of the pathogenic fungus shared 100% similarity with that of Fusarium avenaceum (JN6317483). The morphologic characteristics and ITS sequence analysis all indicated that the pathogenic fungus was F.avenaceum. The suitable culture temperature was from 5 ℃ to 40 ℃, and the optimum was 20 ℃. D fructose and leucine had significant promoting effect on the growth of the mycelia (P<0.05), and chloralose and ammonium carbonate significantly inhibited mycelial growth (P<0.05). Oatmeal medium played a significant role in promoting mycelial growth (P<0.05). |
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