| 王涵琦，畅涛，杨成德*，陈秀蓉.马铃薯炭疽病(Colletotrichum coccodes)拮抗菌株的筛选及鉴定[J].植物保护,2014,40(1):38-42. |
| 马铃薯炭疽病(Colletotrichum coccodes)拮抗菌株的筛选及鉴定 |
| Screening and identification of the antagonistic organisms against Colletotrichum coccodes on potato |
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| DOI： |
| 中文关键词: 炭疽病 生防菌株 筛选 鉴定 |
| 英文关键词:Colletotrichum coccodes biocontrol strain screening identification |
| 基金项目: |
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| 中文摘要: |
| 采用对峙培养法对马铃薯炭疽病的拮抗菌株进行了筛选和鉴定。结果表明，拮抗菌株ZHA10对炭疽病病原菌具有良好的抑制作用，抑制率达76.43%，其可以使病原菌菌丝变形和裂解；ZHA10菌体短杆状、革兰氏阳性、大小为(0.5~0.75)μm × (1.5 ~3.1)μm、芽胞中至尖端生。通过生理生化测定、16S rDNA以及gyrB序列分析，鉴定ZHA10为枯草芽胞杆菌(Bacillus subtilis)。此外，ZHA10还对番茄早疫病菌(Alternaria solani)、马铃薯干腐病菌(Fusarium oxysporum)、孜然根腐病菌(Fusarium solani)、小麦根腐病菌(Bipolaris sorokiniana)、黄瓜枯萎病菌(Fusarium oxysporum)和马铃薯褐腐病菌(Stysanus stemonitis)均具有较强的拮抗作用。ZHA10抑菌能力良好，抑菌谱宽，具有进一步开发应用的潜力。 |
| 英文摘要: |
| In this study, the biocontrol strain of Colletotrichum coccodes on potato was screened by confrontation culture method. The results showed that the strain ZHA10 had good inhibition against C.coccodes, and the inhibition rate reached 76.43%. In order to understand the bacteriostatic mechanism of the endophytic strain ZHA10 to C.coccodes and its taxonomic status, a series of physiological and biochemical experiments were conducted. The results showed that the antibacterial activity was mainly due to hyphal deformation and cracking. ZHA10 was short rod shaped, gram positive, and （0.5-0.75） μm×(1.5-3.1)μm in size, with endospores growing from the center to the tip. Through 16S rDNA and gyrB gene sequence analysis, ZHA10 was identified as Bacillus subtilis. Besides, ZHA10 could effectively inhibit the growth of Alternaria solani, Fusarium oxysporum, Fusarium solani, Bipolaris sorokiniana, Fusarium oxysporum and Stysanus stemonitis, which showed that ZHA10, with broad spectrum and high antagonistic activity against pathogenic fungi, had good potential for development and application. |
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