| 陆继臣1,2，迟乃玉2，张庆芳2*.格氏沙雷菌CNY-04对灰霉病菌作用机制及抑菌物质理化性质研究[J].植物保护,2013,39(3):72-76. |
| 格氏沙雷菌CNY-04对灰霉病菌作用机制及抑菌物质理化性质研究 |
| Antimicrobial activity of Serratia grimesii CNY 04 against Botrytis cinerea and characteristic analysis of antagonistic substances |
| 投稿时间：2014-04-16 修订日期：2014-07-11 |
| DOI： |
| 中文关键词: 格氏沙雷菌 灰霉病菌 抑菌作用 理化性质 |
| 英文关键词:Serratia grimesii Botrytis cinerea antagonistic substance physicochemical property |
| 基金项目:现代农业燕麦荞麦产业技术体系建设专项（CARS-08-C-1） |
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| 中文摘要: |
| 从蔬菜根际土壤中筛选到一株生防格氏沙雷菌（Serratia grimesii）CNY 04，利用抑菌圈法测定其对灰霉病菌（Botrytis cinerea Pers. ex Fr.）的抑制效果，通过抑制孢子萌发、影响菌丝正常生长试验对CNY 04的作用机制进行研究，并对CNY 04菌株相关性状及抑菌物质的理化性质进行了研究。结果表明，CNY 04菌株对灰霉病菌拮抗能力强且遗传稳定，抑菌圈直径达到34 mm；CNY 04可对灰霉病菌菌丝结构造成破坏；可显著抑制灰霉病菌分生孢子萌发，抑制率为99%；CNY 04菌株不产生硝吡咯菌素、氢氰酸及水解酶类，可产生嗜铁素和蛋白酶；抑菌物质具有较好的热稳定性和酸碱稳定性。抑菌物质由灰霉病菌诱导产生，与已报道的沙雷菌属的作用机理完全不同。 |
| 英文摘要: |
| Serratia grimesii CNY 04 was screened from the rhizosphere soil of the vegetable. The antibacterial circle method was used for determination of the activity of CNY 04 against Botrytis cinerea. The study was carried out through the inhibition of spore germination and hyphal growth, and the related traits of S.grimesii CNY 04 were determined. The characteristic analysis of antagonistic substances was also studied. The results showed that the strain CNY 04 exhibited genetic stability and strong antagonistic ability. The antibacterial circle diameter was up to 34 mm. The mycelial structure of B.cinerea could be damaged by CNY 04. The strain CNY 04 significantly inhibited the spore germination of B.cinerea, with an inhibition rate of 99%. CNY 04 could not produce pyrrolnitrin, hydrocyanic acid, and hydrolases, but could produce siderophore and proteinase. The antibacterial material had a high thermal stability and acid based stability. Antibacterial substances were induced by B.cinerea. The bacteriostatic mechanism was completely different from that previously reported. |
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