| 刘爱芬１，２，张鑫３，张玮３，燕继晔３，刘瑞琪４，庞倩茹４，李兴红３，黄金光１，２.葡萄溃疡病菌转化子致病力快速评价体系的建立[J].植物保护,2013,39(2):96-100. |
| 葡萄溃疡病菌转化子致病力快速评价体系的建立 |
| Establishment of a rapid virulence evaluation system in Botryosphaeria rhodina transformants |
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| DOI： |
| 中文关键词: 葡萄溃疡病菌 转化子 致病力 葡萄绿枝条 |
| 英文关键词:Botryosphaeria rhodina transformants virulence grapevine green shoots |
| 基金项目: |
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| 中文摘要: |
| 为探索快速、稳定、高效的葡萄溃疡病菌转化子致病力评价方法,分别采用无伤接种、昆虫针针刺接种、打孔器打孔接种等3种接种方法，将随机选取的来自葡萄溃疡病菌REMI转化子库的50株转化子分别接种''‘富士’苹果果实和‘夏黑’葡萄一年生绿枝条，测量病斑大小。结果表明，用葡萄绿枝条接种能区分不同转化子的致病力，而苹果果实则仅能将致病力减弱的转化子筛选出来。在葡萄绿枝条上致病力减弱的转化子有96.15%在‘富士’苹果果实上的致病力也是减弱的，而用苹果果实接种未筛选到致病力增强转化子。上述结果为获得致病力突变体奠定了基础。 |
| 英文摘要: |
| To explore a rapid, efficient and stable evaluation system for the virulence of Botryosphaeria rhodina transformant, mycelium discs of B.rhodina were inoculated onto apple fruit and grapevine green shoots, which were non wounded and wounded by 5 mm diameter sterilized puncher or insect pins, respectively. Fifty transformants from B.rhodina REMI library were used in this study. Mycelium discs of these fifty transformants were inoculated onto apple fruit and grapevine green shoots, and then the length and width of disease lesion were measured. It showed that the virulence of transformants was significantly different on grapevine green shoots, while there was no significant difference on apple fruit. 96.15% transformants with weakened virulence on the grapevine green shoots also had weakened virulence on ‘Fuji’ apple fruit, while there were no transformants with higher virulence than wild type strain on apple fruit. The above results establish foundation for getting B.rhodina mutants with weak virulence. |
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