| 郭立新1，谭毅1，刘永丰1，邓丛良2，段维军3*，许邹亮4.逆转录环介导等温扩增技术检测南芥菜花叶病毒[J].植物保护,2013,39(2):91-95. |
| 逆转录环介导等温扩增技术检测南芥菜花叶病毒 |
| Detection of Arabis mosaic virus by the reverse transcription Loop mediated isothermal amplification method |
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| DOI： |
| 中文关键词: 南芥菜花叶病毒 逆转录环介导等温扩增 检测 |
| 英文关键词:Arabis mosaic virus reverse transcription loop mediated isothermal amplification (RT-LAMP) detection |
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| 中文摘要: |
| 根据南芥菜花叶病毒外壳蛋白基因序列设计并合成了2组RT-LAMP引物，通过筛选试验，最终确定Ar4组引物为最佳引物，建立了南芥菜花叶病毒的RT-LAMP检测方法。同时，通过实时浊度仪和钙黄绿素分别对检测结果进行了判断。特异性和灵敏度试验结果显示，RT-LAMP方法快速、特异且灵敏，其灵敏度与普通RT-PCR法一致。 |
| 英文摘要: |
| Two sets of primers were designed and synthesized based on coat protein gene of Arabis mosaic virus (ArMV) for reverse transcription loop mediated isothermal amplification (RT LAMP) assay. Based on primer screening, the set Ar4 was selected as the optimal primers. A simple and sensitive RT LAMP method was established to detect ArMV. Amplification products were detected by the real time turbidimeter and using calcein separately. Specificity and sensitivity assay showed that the RT-LAMP detection was rapid, specific and sensitive, and it was the same sensitive as RT-PCR. |
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