| The composition of artificial diet for Apolygus lucorum was further optimized using orthogonal design with 3 factors and 2 levels. The optimized artificial diet E was comprised of sucrose 2.80 g, brewer’s yeast 0.25 g, 50% honey solution 2.50 g, whole chicken egg 22.50 g, toasted wheat germ 10.00 g, lima bean meal 30.00 g, toasted soybean flour 2.50 g, egg yolk 30.00 g, soybean lecithin 2.25 g, Vanderzant vitamin mixture 1.80 g, 10% acetic acid 0.50 mL, streptomycin sulphate 0.005 g, cefadroxil 0.005 g, formalin 0.10 mL, propionic acid 0.10 mL and sterilized water 164.00 g. When A. lucorum was reared individually on the optimized artificial diet, the nymph survival rate, developmental duration and fecundity were 81.25%, 11.68d and 32.36 eggs, respectively, which were not significantly different from those on green beans. The optimized diet shortened the developmental duration of nymphs, but the survival rate of mass reared nymphs on the optimized diet was only 55.74%, significantly lower than that (73.77%) on green beans. Range analysis for orthogonal design indicated the importance of three components, judged by the influence on the nymph survival rate, developmental duration and fecundity, was Vanderzant vitamin mix > soybean lecithin ≥ sucrose. The further optimization for the artificial diet of A. lucorum was also discussed. |
