| 杜蕙1，漆永红1，吕和平1，陈书龙2，陈明1*.甘肃省保护地蔬菜根结线虫种类鉴定及其rDNA-ITS序列分析[J].植物保护,2013,39(1):93-96. |
| 甘肃省保护地蔬菜根结线虫种类鉴定及其rDNA-ITS序列分析 |
| Identification of vegetable root knot nematode from Gansu Province and its rDNA-ITS sequence analysis |
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| DOI： |
| 中文关键词: 蔬菜 根结线虫 核糖体DNA-ITS 种类鉴定 |
| 英文关键词:vegetables root-knot nematode ribosomal DNA-ITS species identification |
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| 中文摘要: |
| 为明确甘肃省保护地蔬菜根结线虫的种类，采用rDNA-ITS-PCR方法结合其2龄幼虫、雌虫会阴花纹等形态学特征对采自甘肃省9个市的主要蔬菜生产基地保护地蔬菜根结线虫的11个种群样本进行了种类鉴定，并对其ITS区域进行序列测定及分析比对。结果表明:11个根结线虫种群的ITS区域序列与GenBank数据库已知南方根结线虫（Meloidogyne incognita）ITS区域序列的相似性达到99%，其ITS 片段大小介于690～695 bp之间,表明甘肃省保护地中蔬菜根结线虫为南方根结线虫。 |
| 英文摘要: |
| To clear the species of vegetable root knot nematodes in Gansu Province, 11 root knot nematode (Meloidogyne sp.) populations collected from greenhouse vegetables in Gansu Province were identified based on rDNA-ITS sequence analysis and the perineal pattern of female nematodes. The results indicated that similarity between rDNA-ITS sequences of 11 root knot nematode samples and M.incognita species from GenBank database reached 99%, and its sequence length were about 690-695 bp, indicating that the vegetable root knot nematodes in Gansu Province belong to Meloidogyne incognita. |
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