| 赵李霞，刘程国，付颖，高爽，叶非*.安全剂R-28725保护玉米免受氯磺隆药害的作用[J].植物保护,2013,39(1):29-32. |
| 安全剂R-28725保护玉米免受氯磺隆药害的作用 |
| The effects of the herbicide safener R 28725 in protecting maize from the phytotoxicity of the herbicide chlorsulfuron |
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| DOI： |
| 中文关键词: R-28725 氯磺隆 GSH ALS GST |
| 英文关键词:R-28725 chlorsulfuron GSH ALS GST |
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| 中文摘要: |
| 采用生物测定法研究了安全剂R-28725保护玉米免受除草剂氯磺隆药害的作用。在氯磺隆毒土浓度为2 μg/kg时，使用5 mg/kg R-28725浸种处理，玉米根部受到氯磺隆抑制的谷胱甘肽（GSH）含量由空白对照的573%恢复至83.4%；玉米叶片中乙酰乳酸合成酶（ALS）活性增加了17.2%；玉米根部的谷胱甘肽S-转移酶（GST）活性增加了191.8%；GST酶促反应动力学参数V-max增大102.5%，Km减小40.0%。结果显示：R-28725能够提高氯磺隆处理后玉米中GSH的含量，增加GST和ALS活性，增强了玉米根部GST酶对底物的亲和力，缓解氯磺隆对玉米产生的药害。 |
| 英文摘要: |
| Physiological and biochemical tests were conducted to study the protective effects of the herbicide safener R 28725 in reducing the phytotoxicity of chlorsulfuron to maize. The GSH content in maize roots was increased from 57.3% to 83.4%, the ALS activity in shoots was increased by 17.2%, and the GST activity in roots was increased by 191.8%, when 2 μg/kg chlorsulfuron was used in soils and the seeds were soaked in 5 mg/kg R 28725. When treated only by R-28725, the kinetic parameters V-max of GST was increased by 102.5%, and Km was decreased by 40.0%. The results suggested that R 28725 could improve the GSH content, GST activity, and ALS activity. The maize could be restored from the injury of chlorsulfuron by enhancing GST affinity for the CDNB substrate. |
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