| 徐薪惟，李景富，姜景彬，张贺，康立功，陈秀玲，王傲雪，许向阳*.番茄抗枯萎病I2基因的SNP分型[J].植物保护,2012,38(6):22-26. |
| 番茄抗枯萎病I2基因的SNP分型 |
| SNP genotyping of Fusarium wilt resistance gene I 2 in tomato |
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| DOI： |
| 中文关键词: 番茄 枯萎病 I 2基因 SNP 等位基因特异PCR（AS-PCR） |
| 英文关键词:tomato Fusarium wilt I 2 SNP allele specific polymerase chain reaction (AS-PCR) |
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| 中文摘要: |
| 利用直接测序方法检测番茄抗感品种I2基因的DNA序列，发现了2个SNPs。以其为3′端，设计等位基因特异引物及其互补引物，研究了特异引物3′端碱基错配类型和位置对等位基因特异PCR的影响，并对52份种质资源进行了SNP分型。结果表明在下游引物3′末端第1、2位引入错配碱基可以提高反应的准确性；引入C T碱基错配的引物，退火温度在58 ℃时能把I2基因的突变位点和感病品种的对应位点区分开来，为番茄抗枯萎病辅助育种提供了有力工具。 |
| 英文摘要: |
| Two SNPs were discovered by aligning the I 2 genes between resistant varieties and susceptible varieties. Allele specific primers and their complementary primers were designed using the SNPs as their 3′ end. In addition, we studied the effect of the mismatched bases at the 3′ end of the allele specific primers on PCR. To type these SNPs, 52 groups SNP genotypes were studied, with SNPs detected in tomato I 2gene. The results showed that introducing proper mismatched bases at the first or the second sites of the 3′ end of the antiprimer could improve the accuracy of the reaction, and the primer with C T mismatch could distinguish mutation location of I 2from its susceptible allele location at the annealing temperature 58 ℃. The results provided a powerful tool for Fusarium wilt resistance gene selection in tomato breeding program. |
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