| 肖琼1,2,杨海芳3,艾洪木1,周卫川2*.花园葱蜗牛形态学与PCR检测鉴定技术[J].植物保护,2012,38(5):108-111. |
| 花园葱蜗牛形态学与PCR检测鉴定技术 |
| Morphology and PCR identification technology of Cepaea hortensis |
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| DOI： |
| 中文关键词: 花园葱蜗牛 植物检疫 PCR 鉴定 |
| 英文关键词:Cepaea hortensis plant quarantine PCR identification |
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| 中文摘要: |
| 花园葱蜗牛（Cepaea hortensis Müller）是我国新增的进境植物检疫性有害生物名录中的成员，该螺与近似种在贝壳形态上难以鉴别。本文应用贝壳、软体解剖和PCR技术相结合的方法，研究了花园葱蜗牛的检测鉴定技术，结果表明，PCR方法特异性强、灵敏度高、检测结果稳定，并与形态学鉴定结果一致，该方法为非软体动物分类专业的检疫人员检测鉴定花园葱蜗牛提供了快速简便的技术方法。 |
| 英文摘要: |
| Cepaea hortensis Müller is one of the species in the import plant quarantine pest list. This species is difficult to be distinguished from similar species by the shell characters. In this study, the shell characters, anatomical method and PCR were used to identify C. Hortensis. The results showed that PCR method had high specificity, sensitivity and stability, and it could provide a quick and easy technical method for identification of C. hortensis. |
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