| 王聪1,刘颖超1*,庞民好1,董金皋2*.高效液相色谱柱后衍生法测定农田沟渠水中草甘膦残留[J].植物保护,2012,38(5):96-99. |
| 高效液相色谱柱后衍生法测定农田沟渠水中草甘膦残留 |
| Determination of glyphosate in the water of farmland ditch by high performance liquid chromatography-post column derivation |
| |
| DOI： |
| 中文关键词: 高效液相色谱 柱后衍生 农田沟渠水 草甘膦 |
| 英文关键词:high performance liquid chromatography post column derivation the water of farmland ditch glyphosate |
| 基金项目: |
|
| 摘要点击次数: 3120 |
| 全文下载次数: 1750 |
| 中文摘要: |
| 为了准确评价草甘膦在农田沟渠水中使用后其对生态环境的安全性，建立了高效液相色谱（HPLC）-柱后衍生法对农田沟渠水中草甘膦残留量的检测方法。将田间沟渠水样中草甘膦经次氯酸钠和邻苯二甲醛、巯基乙醇衍生化后，采用荧光检测器检测。衍生剂1(5%)次氯酸钠添加量100 μL/L；衍生剂2硼酸钾和OPA添加量分别是130 g/L和100 mg/950 mL。草甘膦在0.05～2 mg/L范围内线性良好，相关系数为0.999 9。在农田沟渠水中，草甘膦3个添加水平（0.05、0.5、1 mg/L）的平均回收率（n=5）为98.2%～104.4%，5次测量的相对标准偏差为2.1%～3.6%，方法检出限为0.05 mg/L。该方法灵敏、简便、快速，适合于农田沟渠水中草甘膦残留量的检测。 |
| 英文摘要: |
| In order to accurately evaluate the bio-environmental safety of glyphosate, a HPLC post column derivation method was developed to determine glyphosate in the water of farmland ditch. Glyphosate in the water of farmland ditch was derivatized with the sodium hypochlorite, o-phthalic aldehyde and mercaptoetha-nol, which were detected with uorescence detector. The 5% sodium hypochlorite volume of derivative 1 was 100 μL/L; potassium borate and OPA volumes of derivative 2 were 130 g/L and 100 mg/950 mL, respectively. The linearities of glyphosate were at the concentration range of 0.05 to 2.0 mg/L, with a correlation coefficient of 0.999 9. The mean spiked recoveries of glyphosate at 3 spiked levels ranged from 98.2% to 104.4%. The relative standard deviations（RSD, n=5）were 2.1%-3.6%. The limit of detection was 0.05 mg/L. This method is sensitive, simple, rapid and suitable for determining glyphosate in the water of farmland ditch. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
