| 马丽娟1，滕忠才2，臧欢1，刘廷辉1，李瑞军1*，郭巍1，巩兰菊3.对斜纹夜蛾高效绿僵菌的筛选[J].植物保护,2012,38(5):78-83. |
| 对斜纹夜蛾高效绿僵菌的筛选 |
| Screening of high virulence strains of Metarhizium anisopliae against Spodoptera litura |
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| DOI： |
| 中文关键词: 金龟子绿僵菌 斜纹夜蛾 生物测定 筛选 |
| 英文关键词:Metarhizium anisopliae Spodoptera litura bioassay screen |
| 基金项目: |
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| 摘要点击次数: 1550 |
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| 中文摘要: |
| 对从土壤中分离到的金龟子绿僵菌中生长性状较好的6个菌株，通过测定对黄粉虫幼虫的僵虫率和致死中时，选出3株致病效果好的菌株M7-9、M7-(16-18)、M7-(64-65)，再采用点滴法接种对斜纹夜蛾进行毒力测定。结果表明，不同菌株对斜纹夜蛾的毒力与菌株种类有密切关系，3株菌株对斜纹夜蛾的LT50分别为2.70、3.07、5.79 d。以此3株菌进行抗高温及抗紫外线试验，菌株M7-9和M7-(16-18)对高温和紫外线都具有较好的抗性，且M7-9对紫外线的抗性强于M7-(16-18)，两菌株均具有进一步研究的价值。 |
| 英文摘要: |
| Six strains of Metarhizium anisopliae with good growth traits, isolated from soil in Baoding area, were screened against Tenebrio molitor Linnaeus by bioassay. Among them, M7-9, M7-(16-18)and M7-(64-65)strains, which showed higher virulence than other isolates, were selected to test their pathogenicity against Spodoptera litura(Fabricius). The LT50 of strain M7-9, M7-(16-18) and M7-(64-65) against S. litura were 2.70, 3.07 and 5.79 d, respectively. The 3 strains were then treated with the ultraviolet ray and heat. The results demonstrated that the resistance of M7-9 and M7-(16-18) to high temperature and ultraviolet ray was higher，and the resistance of M7-9 to the ultraviolet ray was higher than that of M7-(16-18). These results suggested that strains M7-9 and M7-(16-18) have better exploitation and application value in the future. |
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