| 谭海文1，卢燕回2，王雅1，陈振东1，袁高庆1，林纬1，黎起秦1*.广西烤烟棒孢霉叶斑病病原分子鉴定及其生物学特性补充[J].植物保护,2012,38(5):35-39. |
| 广西烤烟棒孢霉叶斑病病原分子鉴定及其生物学特性补充 |
| Molecular identification and a supplement to the biological characteristics of the pathogen causing flue-cured tobacco corynespora leaf spot in Guangxi |
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| DOI： |
| 中文关键词: 烤烟棒孢霉叶斑病 山扁豆生棒孢 鉴定 生物学特性 |
| 英文关键词:Flue-cured tobacco corynespora leaf spot Corynespora cassiicola identification biological characteristics |
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| 中文摘要: |
| 本文对广西烤烟棒孢霉叶斑病的病原进行鉴定,并对其生物学特性进行初步研究。根据该病原菌的形态特征、致病性及rDNA-ITS序列测定，将引起广西烤烟棒孢霉叶斑病的病原菌鉴定为山扁豆生棒孢(Corynespora cassiicola)。生物学特性测定结果表明：27.5 ℃、培养初始pH为6、以麦芽糖作为C源、硝酸钾作为N源及完全光照等条件最适合其菌丝生长；30 ℃、以乳糖作为C源、硝酸钾作为N源及完全黑暗等条件最有利于该菌分生孢子形成；27.5 ℃、湿度为100%+水膜、12 h光暗交替等条件最有利于分生孢子萌发；分生孢子的致死温度为52 ℃。这是该病在广西的首次报道。 |
| 英文摘要: |
| The identification of the pathogen causing flue-cured tobacco corynespora leaf spot in Guangxi and its biological characteristics were preliminarily studied. The pathogen of flue-cured tobacco corynespora leaf spot was identified as C. cassiicola based on its morphology, pathogenicity and the analysis of sequences of ribosomal rDNA-ITS. The optimum temperature, pH value, carbon source, nitrogen source and light treatment for the mycelial growth of C. cassiicola were 27.5 ℃, 6, maltose, potassium nitrate and full light, respectively. For the sporulation, the optimum temperature was 30 ℃, and the best carbon/ nitrogen source was lactose/ potassium nitrate, and full darkness was the most suitable light treatment. For conidial germination , the most favorite temperature, relative humidity and light treatment were 27.5 ℃, 100% and 12 h alternate light, respectively. The lethal temperature for the spores was 52 ℃. It is the first report of the tobacco disease in Guangxi, China. |
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