| 张亮,袁争,朱蔚,高旭晖*.4种植物提取物对茶炭疽病菌的体外抑制作用[J].植物保护,2012,38(4):137-140. |
| 4种植物提取物对茶炭疽病菌的体外抑制作用 |
| Inhibition of 4 plant extracts against Gloeosporium theae sinesis in vitro |
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| DOI： |
| 中文关键词: 植物提取物 茶炭疽病菌 抑菌作用 孢子萌发 |
| 英文关键词:plant extract Gloeosporium theae-sinesis inhibition spore germination |
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| 中文摘要: |
| 以丙酮作溶剂对芦柑皮、艾蒿、香樟叶和侧柏叶进行浸提；采用菌丝生长速率法测定各提取物对茶炭疽病菌菌丝的抑制作用，采用水琼脂培养法测定提取物对分生孢子萌发的抑制效果。结果表明:1 500 μg/mL浓度下，各提取物对茶炭疽病菌菌丝均有抑制作用，其中芦柑皮96 h的抑制率达到93.32%，艾蒿、香樟叶和侧柏叶96 h的抑制率分别为61.10%、55.64%和48.20%，EC50分别为8.981、839.575、1 058.682 μg/mL和1 429.094 μg/mL；芦柑皮、艾蒿、香樟叶和侧柏叶提取物对分生孢子的抑制率分别为34.85%、85.54%、55.40%和59.68%，EC50分别为5 764.142、281.121、1 502.49 μg/mL和969.369 μg/mL。芦柑皮、艾蒿丙酮提取物具有开发植物源杀菌剂的潜力。 |
| 英文摘要: |
| This study was to test the inhibitory effects of 4 plant extracts on Gloeosporium theae-sinesis Miyake in vitro.Citrus madurensis peels, Artemisia argyi plant, Cinnamomum camphora leaves and Platycladus orientalis leaves were extracted by acetone; Mycelium growth rate methods and water agar culture were applied to determine the inhibitory effects of these extracts against mycelium growth and spore germination of G.theae-sinesis, respectively. All extracts had effects on mycelium growth. After treatment for 96 h, the inhibition rates of C.madurensis peels, A.argyi plant, C.camphora leaves and P.orientalis leaves with dosage of 1 500 μg/mL were 93.32%, 61.10%, 55.64% and 48.20% , respectively, while their EC50 values were 8.981 μg/mL, 839.575 μg/mL, 1 058.682 μg/mL and 1 429.094 μg/mL, respectively; Their inhibition rates against spore germination were 34.85%, 85.54%, 55.40% and 59.68%, respectively, and EC50 values were 5 764.142, 281.121, 1 502.49 μg/mL and 969.369 μg/mL, respectively. The acetone extracts of C.madurensis peels and A.argyi plant had great potential for the development of botanical fungicides. |
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