| 孙丽娜,芮昌辉,袁会珠*.小菜蛾鱼尼丁受体基因克隆及序列分析[J].植物保护,2012,38(4):118-123. |
| 小菜蛾鱼尼丁受体基因克隆及序列分析 |
| Molecular cloning and sequence analysis of ryanodine receptor gene in the diamondback moth, Plutella xylostella |
| |
| DOI： |
| 中文关键词: 小菜蛾 鱼尼丁受体 基因克隆 |
| 英文关键词:Plutella xylostella ryanodine receptor molecular cloning |
| 基金项目: |
|
| 摘要点击次数: 1100 |
| 全文下载次数: 2044 |
| 中文摘要: |
| 利用RT-PCR和RACE技术克隆了小菜蛾(Plutella xylostella)鱼尼丁受体（Px-RyR）基因，其核苷酸序列全长15 748 bp，5′非编码区267 bp，3′端非编码区109 bp，开放阅读区全长为15 372 bp（GenBank登录号：JF927 788），编码5 123个氨基酸残基。估测其蛋白分子量为579.39 ku，等电点为5.45。该基因编码氨基酸序列和其他鳞翅目昆虫RyR氨基酸序列比对相似性较高（92%），与哺乳动物3种亚型RyRs的相似性为45%~47%。此外，二级结构预测，其C-末端存在6个跨膜区域；且Px-RyR中存在一个出现4次重复，长度为89~95个氨基酸的RyR结构域，平均相似性为33%。 |
| 英文摘要: |
| The full-length cDNA of the ryanodine receptor from the lepidopteran pest Plutella xylostella (Px-RyR) was 15 748 bp, containing a 5′-UTR of 267 bp, a 3′-UTR of 109 bp and a full open reading frame (ORF) of 15 372 bp (GenBank accession No. JF927 788). The amino acid sequence deduced from the sequence was 5 123 amino acids. The calculated molecular weight for Px-RyR was 579.39 ku, and its isoelectric point was 5.45. The deduced amino acids sequence of Px-RyR showed a high level of similarity with the RyRs sequences from other Lepidoptera insects, such as the tobacco budworm Heliothis virescens (92%) and silkworm Bombyx mori (92%). It shared 45%-47% similarity with the vertebrate isoforms. In addition, there were six essential transmembrane (TM) segments, predicted from the secondary structure of the deduced amino acid sequence at C-terminal of Px-RyR. A repeated sequence motif of 89-95 amino acids was observed, sharing 33% similarity on average and occurring four times. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
