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欧善生1,2,梁沛2,宋敦伦2,史雪岩2,高希武2*.氯虫苯甲酰胺亚致死剂量对棉铃虫生长发育和解毒酶活性的影响[J].植物保护,2012,38(4):1-8.

氯虫苯甲酰胺亚致死剂量对棉铃虫生长发育和解毒酶活性的影响

Effects of sublethal dosage of chlorantraniliprole on development and detoxifying enzymes activity of Helicoverpa armigera

DOI：

中文关键词: 氯虫苯甲酰胺棉铃虫亚致死剂量生长发育解毒酶

英文关键词:chlorantraniliprole Helicoverpa armigera sublethal dosage growth detoxifying enzyme

基金项目:

作者	单位
欧善生1,2,梁沛2,宋敦伦2,史雪岩2,高希武2*	1. 广西农业职业技术学院，南宁530007； 2. 中国农业大学农学与生物技术学院，北京100193

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中文摘要:

在室内采用培养基混药法测定氯虫苯甲酰胺亚致死剂量(LC10、LC25和LC50)对棉铃虫生长发育和体内3种解毒酶系的影响。结果表明：各剂量处理3龄幼虫48 h后饲养，各处理组当代(F0)3～6龄幼虫历期均比对照组延长0.2～3.8 d，但成虫平均历期比对照组缩短0.8～2.5 d，子代（F1）1～6龄幼虫历期均比对照组延长0.2～2.6 d，成虫平均历期比对照组缩短0.1～0.5 d；各剂量处理后，各处理组F0代3～6龄幼虫和蛹的体重、F1代4～6龄幼虫和蛹的体重均比对照组减轻，且剂量越大，体重减轻越显著；各处理组F0代种群趋势指数分别比对照组下降了16.27%、58.51%和71.17%，F1代分别下降了13.66%、52.61%和66.63%；各处理组F0代3～6龄幼虫体内的羧酸酯酶(CarE)和细胞色素P450O脱乙基酶(P450-ECOD)比活力均高于对照组，其中6龄幼虫这两种酶比活力分别比对照组提高了5%～16%和23%～66%，但各处理组谷胱甘肽硫转移酶(GSTs)比活力均低于对照组，其中6龄幼虫GSTs比活力分别比对照组降低了16.23%、58.51%和71.17%。表明在LC10～LC50剂量范围内，氯虫苯甲酰胺处理棉铃虫3龄幼虫的亚致死剂量越高，其生长发育受抑制越显著，P450-ECOD和CarＥ比活力上升越高，GSTs比活力下降也越大。

英文摘要:

This research, by blending the targeted chemical into culture medium, aims to study the effects of three sub lethal doses(i.e.LC10,LC25,and LC50)of chlorantraniliprole on the development and the activities of three detoxifying enzymes of Helicoverpa armigera Hübner. The results showed that, after treatment with three different sub lethal doses to the third instar larvae for 48 hours, the development durations of the treated third to sixth instar larvae (F0) were 0.2-3.8 d longer than that of the control, and the average development durations of the adults were 0.8-2.5 d shorter than that of the control; the durations of 1st -6th instar larvae of the offspring (F1) were lengthened by 0.2-2.6 d compared with the control; the average duration of the adult was shortened by 0.1-0.5 d compared with the control. Compared with the control, the weights of the third instar to sixth instar larvae and their pupae (F0) and the fourth to sixth instar larvae and the pupae (F1) were decreased, which was correlated with the dose negatively; the population trend indexes of the groups treated with LC10, LC25, and LC50 decreased by 16.27%, 58.51% and 71.17% in F0 and 13.66%, 52.61% and 66.63% in F1,respectively; by comparison with the control, the activities of CarE and P450 ECOD of the third instar to sixth instar larvae (F0) were significantly higher, especially in the sixth instar larvae, the activities of the two detoxification enzymes were strengthened by 5%-16% and 23%-66%, respectively. In contrast, the activities of GSTs of the groups treated with three different sub lethal doses were lower than that of the control, and in the sixth instar larvae, the activity decreased by 16.23%, 58.51% and 71.17%, respectively. This suggest that, within the range from LC10 to LC50, the higher the sub lethal doses of chlorantraniliprole treating the third instar H. armigera larvae, the more significant the suppression effect on the development is, and the higher the activities of CarE and P450 ECOD, the lower the activity of GSTs.

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