| 张雅涵，茆振川*，凌键，陈国华，杨宇红，谢丙炎.黄瓜根结发育早期WRKY基因的分离[J].植物保护,2012,38(3):13-17. |
| 黄瓜根结发育早期WRKY基因的分离 |
| Isolation of cucumber WRKY genes during the early stage of root knot development |
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| DOI： |
| 中文关键词: 根结线虫 黄瓜 WRKY 分离 |
| 英文关键词:Meloidogyne incognita Cucumis sativus WRKY gene gene isolation |
| 基金项目: |
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| 摘要点击次数: 1040 |
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| 中文摘要: |
| 为了确定黄瓜根结线虫侵染早期与取食位点形成相关的WRKY基因, 以根结线虫侵染早期的黄瓜根为材料，基于黄瓜全基因组WRKY基因预测，进行RT PCR扩增。在根结线虫侵染早期的黄瓜根组织中共分离到24个WRKY基因, 分别定位于黄瓜的7条染色体上。系统进化分析表明，在分离到的WRKY基因中有17个为第二家族，6个为第一家族，1个属于第三家族。初步确定8个WRKY基因为根结线虫诱导表达基因，推测黄瓜根结线虫侵染早期为涉及多个WRKY基因参与的复杂调控体系，为进一步研究黄瓜根结线虫取食位点发育机制奠定了基础。 |
| 英文摘要: |
| To isolate the WRKY genes from the cucumber root tips infected by root knot nematode based on the genomic sequence of Cucumis sativus Linn., 24 CsWRKY genes were isolated from the cucumber roots inoculated with Meloidogyne incognita, using RT PCR method. These genes were located in 7 chromosomes of cucumber respectively. The phylogenetic analysis showed that the 24 CsWRKY genes were classified into three subfamilies, among which 17 genes belonged to group Ⅱ, 6 belonged to group Ⅰ, and 1 belonged to group Ⅲ. We speculated that 8 CsWRKY genes were induced by root knot nematode. These suggested that feeding site development of the root knot nematode was a complex system. It was regulated by some WRKY genes. The isolation and characters of these genes was a basis for the further research on the mechanism of nematode feeding site development. |
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