| 王晓华1,张振臣1,2*,乔奇1,2，秦艳红1,2，张德胜1,2,田雨婷1,2.甘薯羽状斑驳病毒外壳蛋白基因的分子变异[J].植物保护,2012,38(2):114-116. |
| 甘薯羽状斑驳病毒外壳蛋白基因的分子变异 |
| Molecular variation of Sweet potato feathery mottle virus coat protein gene |
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| DOI： |
| 中文关键词: 甘薯羽状斑驳病毒 外壳蛋白基因 分子变异 SSCP分析 |
| 英文关键词:SPFMV coat protein gene molecular variation SSCP analysis |
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| 中文摘要: |
| 应用单链构象多态性 (single-strand conformation polymorphism, SSCP)技术结合核苷酸序列测定的方法，对我国甘薯主产区11个省份的甘薯羽状斑驳病毒(Sweet potato feathery mottle virus, SPFMV)外壳蛋白（CP）基因的分子变异情况进行了研究。结果表明，SPFMV CP基因的RT-PCR产物表现了较丰富的图谱类型，50个分离物共产生9种主要的SSCP带型；对显示不同带型的20个样品的CP基因进行了序列测定和进化树分析，CP基因核苷酸序列一致性为77.2%～99.9%。说明这些样品的SPFMV的CP基因存在较大的分子变异，可划分为EA、RC、O和C 4个株系。 |
| 英文摘要: |
| Virus samples were collected from 11 provinces in China, and the molecular variation of Sweet potato feathery mottle virus（SPFMV）coat protein (CP) gene were analyzed using single-strand conformation polymorphism (SSCP) and sequence analysis. SSCP analysis of 50 isolates showed that these isolates produced 9 kinds of SSCP band forms. The results of sequence comparison and phylogenetic analysis showed that the identities of the nucleotide sequences of CP genes among these isolates were 77.2%-99.9%. SPFMV CP genes of different isolates in China had great molecular variability and could be divided into four diverged groups at least, including the East Africa (EA), Russet Crack (RC), Ordinary (O) and Common (C) strain groups. |
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