| 熊琦1,朱永敏1,曹天文2,李捷2,薛皎亮1*,谢映平1,王全亮3,杜仙当4.桃小食心虫病原菌—球孢白僵菌TST05菌株生物学特性研究[J].植物保护,2012,38(2):87-91. |
| 桃小食心虫病原菌—球孢白僵菌TST05菌株生物学特性研究 |
| Biological characteristics of the entomopathogenic fungus of Carposina sasakii, Beauveria bassiana strain TST05 |
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| DOI： |
| 中文关键词: 球孢白僵菌 生物学特性 桃小食心虫 温度和湿度 |
| 英文关键词:Beauveria bassiana biological characteristics Carposina sasakii temperature and humidity |
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| 中文摘要: |
| ［目的］ 研究从自然染病的桃小食心虫幼虫上分离的球孢白僵菌TST05菌株的生物学特性。［方法］ 测定不同培养基、外界不同温度和湿度对该菌株菌丝营养生长及孢子萌发的影响。［结果］ 该菌株在PDA、PPDA、SDAY、SMAY 4种培养基上均生长良好，菌落厚而致密，产孢量均大于3.95×107孢子/mL；菌株适应的温度和湿度范围宽，15~30 ℃之间，RH 30%~100%之间孢子均可萌发、生长和产孢。随着温度接近25 ℃、湿度增大，孢子的萌发率、菌落的生长速率和产孢量都显著增加。15 ℃、RH 100%时产孢量为1.32×107孢子/mL；25 ℃、RH 30%时，产孢量也能达到1.37×107孢子/mL；25 ℃、RH 100%时产孢量达到6.19×107孢子/mL。15 ℃、RH 100%孢子萌发率为52.28%；25 ℃、RH＞80%时，孢子萌发率都能达到90%以上。［结论］ TST05菌株能适应北方干旱低温条件，可开发成为防治桃小食心虫的生物制剂。 |
| 英文摘要: |
| ［Objective］ The biological characteristics of Beauveria bassiana TST05, a strain originally isolated from the naturally infected larvae of Carposina sasakii (Matsumura) (Lepidoptera: Carposinidae), was investigated.［Method］ The effects of culture media, outside temperature and humidity on the mycelium growth, spore germination of the strain were studied.［Result］ The strain could grow well in the five culture media tested,namely PDA, PPDA, SDAY and SMAY. The colonies were sick and dense, and the spore yield exceeded 3.95×107spores/mL in these culture media. The TST05 strain was adapt to a wide range of temperature and humidity. The spore germination, mycelium growth and sporulation were normal at 15-30 ℃ and RH 30%-100%. With the temperature approaching to 25 ℃ and the humidity increased, the rate of spore germination and mycelium growth and the yield of spore were all visibly increasing. The spore yield was 1.32×107spores/mL under 15 ℃ and RH 100%; 1.37×107spores/mL under 25 ℃ and RH 30%, and 6.19×107spores/mL under 25 ℃and RH 100%. The rate of spore germination was 52.28% under 15 ℃ and RH 100%, and over 90% under 25 ℃ and RH＞80%. ［Conclusion］ The strain TST05 was adapt to low temperature and humidity in the north and could become a biological control agent of C. sasakii. |
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