| 赵忠伟1，2，曹广春1，徐光青3，吴乐年4，张泽华1*.昆虫生长调节剂和绿僵菌对意大利蝗几丁质酶活力的影响[J].植物保护,2012,38(2):83-86. |
| 昆虫生长调节剂和绿僵菌对意大利蝗几丁质酶活力的影响 |
| The effect of insect growth regulators and Metarhizium anisopliae on the Calliptamus italicus chitinase |
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| DOI： |
| 中文关键词: 意大利蝗 昆虫生长调节剂 绿僵菌 几丁质酶 |
| 英文关键词:Calliptamus italicus insect growth regulator Metarhizium anisopliae chitinase |
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| 中文摘要: |
| ［目的］ 探明3种昆虫生长调节剂（苯氧威、氟虫脲、灭幼脲）和一种生物农药（绿僵菌189菌株）对意大利蝗3龄蝗蝻几丁质酶活力的影响。［方法］ 用药剂处理3龄意大利蝗蝗蝻，用分光光度法测定几丁质酶分解几丁质产物N乙酰氨基葡萄糖的含量。［结果］ 3种昆虫生长调节剂浓度达到50mg/L时对几丁质酶的抑制作用超过200%；绿僵菌189菌株对意大利蝗几丁质酶活力也有一定程度的抑制，且抑制作用随着孢子浓度的增加而增大，当孢子浓度达到5×107时，意大利蝗几丁质酶的活力仅为3.8U。［结论］ 昆虫生长调节剂和绿僵菌均抑制意大利蝗几丁质酶活力。 |
| 英文摘要: |
| ［Objective］The effect of three insect growth regulators (fenoxycarb, flufenoxuron, diflubenzuron) and a biological pesticide (189 strains of Metarhizium anisopliae〖WTBZ〗) on the chitinase of the 3rd instars of Calliptamus italicus was studied. ［Method］ The third instars of the C. italicus were treated by three pesticides and M. anisopliae, respectively. The content of N-acetyl glucosamine produced by chitin decomposition with chitinase was tested by spectrophotometer.［Result］ The results showed that all of the four pesticides inhibited chitinase activity. When the concentration of each of the three insect growth regulators reached to 50mg/L, their inhibitory effects on the chitinases were more than 200%. 189 strains of M. anisopliae had some inhibitory effects on chitinase of C. italicus. When the spores reached the highest concentration of 5 × 107, the chitinase activity had only 3.8U. ［Conclusion］ Both insect growth regulators and M. anisopliae can inhibite the chitinase activity of C. italicus. |
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