| 黄胜光，卢兆山，邱世明，奚国华，陆道，钟恒禄.广西防城港首次截获苜蓿黄萎病菌[J].植物保护,2012,38(1):180. |
| 广西防城港首次截获苜蓿黄萎病菌 |
| First interception of Verticillium albo-atrum in Fangchenggang of Guangxi |
| |
| DOI： |
| 中文关键词: |
| 英文关键词:Verticillium albo-atrum inspection interception at port |
| 基金项目: |
|
| 摘要点击次数: 1052 |
| 全文下载次数: 986 |
| 中文摘要: |
| 从一批进境的美国苜蓿草样品中保湿分离得到了3株与苜蓿黄萎病菌(Verticillium albo-atrum)相似的目标菌VA1、VA2、VA3。该目标菌在PDA上生长迅速，菌丝生长0.8～1.0 mm/d,2～3 d即可生成肉眼可见的菌落斑点；菌落为白色圆形，边缘规则呈圆形，菌丝较致密，气生菌丝较少，菌落中心乳白色奶冻状；培养4~5 d即后产生气生典型轮枝状分生孢子梗， 7~8 d后菌落中央表面因产生休眠菌丝开始变成黑褐色至黑色，20 d后菌落的表面和背面大部分均变黑色，仍不产生微菌核和厚垣孢子。该目标菌的纯培养用V.albo-atrum特异引物Vaa1/Vaa2 进行检测，PCR 扩增后得到预期330 bp 的产物片段，产物序列与V.albo-atrum相应序列的相似性为100%。从而鉴定确认该目标菌为苜蓿黄萎病菌V.albo-atrum。 |
| 英文摘要: |
| Three strains of the fungus, namely VA1, VA2 and VA3, were isolated from a batch of alfalfa samples, which were imported from USA and incubated under moist conditions, demonstrating the characters similar to V.albo-atrum. The strains grew rapidly on the PDA, with the colony growing at 0.8〖CD*2〗1.0mm/d. After 2〖CD*2〗3 days, the spot colony could be seen on the PDA. In addition, the colony had a regular and rounded margin with white compact mycelium and fewer aerial mycelia. The target fungus produced typical round aerial branched conidiophores after 4〖CD*2〗5 days, and then became dark brown to black along with production of resting hyphae at the centre of the colony after 7〖CD*2〗8 days, and the color of the surface and most of the back of the colony changed to black 20 days later, but still could not produce micro-sclerotia and chlamydospores. The pure cultures of the target germ were detected by the specific primers Vaa1/Vaa2. As expected, a 330 bp fragment was obtained by PCR amplification. The similarity between the product and corresponding sequence in V.albo-atrum was 100%. Therefore, it was confirmed that the target germ was V.albo-atrum. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
