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卢海博1，2，万树青1*.黄皮素内酯Ⅱ在黄皮植物体的分布及对稗草生化代谢的影响[J].植物保护,2012,38(1):31-34.

黄皮素内酯Ⅱ在黄皮植物体的分布及对稗草生化代谢的影响

The distribution of 2′,3′ epoxyanisolactone in different organ of Clausena lansium（Lour.）Skeels and biochemical and metabolic effect on Echinochloa crusgalli L.

DOI：

中文关键词:

英文关键词:2′,3′epoxyanisolactone amount Echinochloa crusgalli metabolism

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作者	单位
卢海博1，2，万树青1*

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中文摘要:

［目的］测定黄皮素内酯Ⅱ（2′,3′epoxyanisolactone）在黄皮［Clausena lansium（Lour.）Skeels］植株的花、枝叶和果核中的含量，明确其对稗草生化代谢的影响。［方法］采用液相色谱法进行含量的测定，小杯法培养供试稗草。［结果］黄皮素内酯Ⅱ在花粗提物中的含量较少，仅为0.43％，在枝叶和果核中的含量分别为3.33％和3.21％。黄皮素内酯Ⅱ处理稗草植株，处理后稗草根中蛋白含量和氨基酸含量与未经处理存在明显的差异，在50 μg/mL的浓度下，可以显著地降低稗草植株内的蛋白含量和增加植株内的氨基酸含量。当处理浓度为50、25、12.5、6.25、3125 μg/mL时，样品中的含氮量分别为1.402 8、1.317 2、1.250 2、1.128 7、0.903 2 mg/g，比对照0.832 1 mg/g均有所增加。黄皮素内酯Ⅱ处理后，稗草的蛋白含量与对照相比有所下降，处理浓度为50、25、12.5、6.25、3.125 μg/mL时，蛋白含量比对照分别降低75.91%、66.43%、62.43%、58.57%和58.03％。［结论］黄皮素内酯Ⅱ对稗草代谢有一定影响。

英文摘要:

［Objective］ The content of 2′,3′epoxyanisolactone in three different organ of Clausena dunniana were tested, the biochemical and metabolic effect on echinochloa crusgalli L. were investigated. ［Method］The content of 2′,3′epoxyanisolactone were analyzed by HPLC analyzing. bioassay of E.crusgalli was tested by small glass method. ［Result］ The result indicated that there are 0.43% in flower, 3.21% in seeds and 3.33% in the leaf and bark, respectively. The result of assay on metabolism to E.crusgalli indicated that 2′,3′epoxyanisolactone could cause the weakness of vigor and functions of root systems, and the decrease of total amount of protein of E.crusgalli , 2′,3′epoxyanisolactone could increase the quantity of amino acid, with the series of concentration of 2′,3′epoxyanisolactone: 50 μg/mL, 25 μg/mL, 12.5 μg/mL, 6.25 μg/mL and 3.125 μg/mL,· the quantity of amino acid of controlled seeding were 1.402 8 mg/g, 1.317 2 mg/g，1.250 2 mg/g, 1.128 7 mg/g and 0.903 2 mg/g respectively, which was higher than the control at 0.832 1 mg/g. Protein quantity of controlled seeding of E.crusgalli decrease 75.91%, 66.43%, 62.43%, 58.57% and 5803％ compared to the control respectively. ［Conclusion］The result indicated that 2′,3′epoxyanisolactone has influence on E.crusgalli.

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