| 潘哲超1，2，徐进1，顾钢3，吴畏4，许景升1，陈顺辉3，冯洁1*.福建及贵州等地烟草青枯菌系统发育分析[J].植物保护,2012,38(1):18-23. |
| 福建及贵州等地烟草青枯菌系统发育分析 |
| Phylogeny of tobacco Ralstonia solanacearum strains from Fujian and Guizhou Provinces |
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| DOI： |
| 中文关键词: |
| 英文关键词:tobacco Ralstonia solanacearum phylotype sequevar egl |
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| 中文摘要: |
| ［目的］探寻烟草上青枯菌的系统发育。［方法］采用演化型分类框架对福建及贵州等地的62个烟草青枯病菌株进行鉴定分析。［结果］基于内切葡聚糖酶基因系统发育学的分析结果表明：所有参试菌株均归属于青枯菌亚洲分支的4个序列变种，分别为序列变种15、17、34和44；尚未发现归属于美洲或非洲分支的烟草青枯病菌株。其中序列变种15和17为优势菌系，序列变种34的菌株都来自福建省，只发现3个菌株属于序列变种44。基于avrA基因的氨基酸序列比对结果表明4个序列变种的avrA基因都属于RS1000类型。［结论］本研究表明福建及贵州等地烟草上的青枯菌存在一定的遗传分化。 |
| 英文摘要: |
| ［Objective］ The phylogeny of tobacco Ralstonia solanacearum strains from Fujian and Guizhou was studied. ［Method］ Sixty two Chinese R.solanacearum strains isolated from tobacco were analyzed using the phylotype classification schemes. ［Result］ Phylogenetic analysis of endoglucanase gene sequences showed that all isolates belonged to phylotype I, including 4 different sequevars: 15, 17, 34 and 44. All of the 34 sequevar strains were collected from Fujian Province. Only 3 strains were identified as sequevar 44. Alighment analysis of amino acid sequences deduced from avrA gene could not distinguished Chinese R.solanacearum strains from the reference strain RS1000. ［Conclusion］ R.solanacearum strains on tobacco from Fujian and Guizhou had genetic differentiation to some degrees. |
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