| 周新改1，马伟华1*，刘美刚2,杨小红3,王沫1.湖北省棉花主产区烟粉虱生物型分布及系统发育分析[J].植物保护,2011,37(5):72-76. |
| 湖北省棉花主产区烟粉虱生物型分布及系统发育分析 |
| Biotype distribution and phylogenetic analysis of Bemisia tabaci populations in main cotton planting areas in Hubei Province |
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| DOI： |
| 中文关键词: 烟粉虱 生物型 PCR-RFLP 系统发育分析 |
| 英文关键词:Bemisia tabaci biotype PCR-RFLP phylogenetic analysis |
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| 中文摘要: |
| ［目的］ 明确武汉、荆州、孝感、随州、武穴5个棉花主产区烟粉虱生物型组成。［方法］ 通过以线粒体细胞色素氧化酶I（mitochondrial cytochrome oxidase I, mtCOI）为基础的PCR-RFLP和基因测序的方法，鉴定湖北5个棉花主产区烟粉虱地理种群的生物型组成，并分析5个地理种群与其他省种群的系统发育关系。［结果］ Q型烟粉虱为湖北省棉花主产区的优势种群，而武汉温室种群则均为B型烟粉虱，土著生物型ZHJ1型在大田中略有分布；系统发育分析表明，湖北省棉花主产区B型和Q型烟粉虱种群均与中国其他多个省的烟粉虱种群亲缘关系较近。［结论］ 本文研究结果可以为湖北省5个棉花主产区烟粉虱的有效防治提供参考。 |
| 英文摘要: |
| ［Objective］ To understand the biotypes of Bemisia tabaci of cotton in main planting areas of Wuhan, Jingzhou, Xiaogan, Suizhou and Wuxue in Hubei Province. ［Method］ In this study, PCR RFLP of mtCOI and gene sequencing method were used to identify the biotypes of whitefly field populations from main cotton planting areas in Hubei Province, and phylogenetic analysis of them was also conducted. ［Result］ The Q biotype was dominant in the field populations, while the B biotype was dominant in greenhouse populations. In addition, ZHJ1 biotype was also identified in Wuxue population. Phylogenetic analysis showed that populations from main cotton planting areas in Hubei Province were always homologous with populations from most other provinces in China. ［Conclusion］ The results may provide a reference for the control of B. tabaci in Hubei Province. |
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