| 姚赛群1，胡冠芳2，刘敏艳2，张新瑞2*.红蓼种子中杀黏虫活性成分的初步分析[J].植物保护,2011,37(4):101-105. |
| 红蓼种子中杀黏虫活性成分的初步分析 |
| A preliminary analysis of the active ingredients from the seeds of Polygonum orientale against Mythimna separata |
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| DOI： |
| 中文关键词: |
| 英文关键词:seeds of P.orientale Mythimna separata insecticidal activities gas chromatography mass spectrometry (GC MS) |
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| 中文摘要: |
| ［目的］ 对红蓼种子乙酸乙酯萃取物中具有潜在杀虫活性的化合物进行初步分析,为指导开发利用杀虫植物资源提供依据。［方法］ 采用硅胶柱层析和气相色谱 质谱(GC MS)联用法，以5龄黏虫为试虫；对红蓼种子萃取物的有效杀虫活性成分进行分析，应用色谱峰面积归一法测定各组分的相对百分含量。［结果］ 极性（甲醇）组分共分离出37个色谱峰，鉴定了其中的35个组分，占总含量的94.46%。弱极性（二氯甲烷）组分共分离出39个色谱峰，鉴定出39个组分。［结论］ 从成分的化学结构推断,红蓼种子的杀虫活性可能存在于14种成分中，主要成分为酮、醇、开环萜和五环三萜烷类化合物。 |
| 英文摘要: |
| ［Objective］ Active ingredients of the ethyl acetate fraction from seeds of Polygonum orientale Linn. were analyzed to provide the basis for the exploitation of the insecticidal plant resources. ［Method］ Insecticidal ingredients from the seeds of P. orientale fractions were analyzed against the 5th instar larvae of Mythimna separata Walker by using silica gel column chromatographer (CC) and gas chromatography mass spectrometry (GC MS). The relative percentage of each composition was determined by area normalization. ［Result］ Thirty seven peaks were found, 35 of which were identified, representing 94.46% of the total contents in polar fraction. Thirty nine peaks were found, all of which were identified in low polar fraction. ［Conclusion］ It was deduced that the insecticidal active ingredients in P. orientale seeds were 14 chemical compounds, belonging to ketone, alcohol, terpene with open ring and pentacyclic triterpene classes. |
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