高艳玲,张威,白艳菊*,申宇,范国权,耿宏伟,刘玮婷.马铃薯主产区病毒病发生情况调查分析[J].植物保护,2011,37(3):149-151. |
马铃薯主产区病毒病发生情况调查分析 |
Investigation and analysis of viral diseases in main potato production areas |
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DOI: |
中文关键词: 马铃薯病毒 普查 DAS-ELISA |
英文关键词:potato viruses investigation DAS-ELISA |
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中文摘要: |
在我国马铃薯主产区黑龙江、内蒙古、甘肃、贵州采集了752份具有典型病毒病症状的样品和疑似样品,应用DAS ELISA方法进行检测。主要筛查6种病毒:PVX、PVY、PVS、PLRV、PVM、PVA。结果显示: 270份样品检测到病毒,总体上,PVY的检出率最高,PVS次之,33份样品是由多种病毒混合侵染造成的,田间PVY+PLRV混合侵染率最高,PVS+PVY次之。试管苗PVS病毒发生较多,原原种和大田种薯PVY病毒发生比例最高。 |
英文摘要: |
Seven hundred and fifty two samples were collected in main potato production areas of China, including Heilongjiang, Inner Mongolia, Gansu and Guizhou. Six viruses, namely PVX, PVY, PVS, PVM, PVA and PLRV, were detected by DAS ELISA. Two hundred and seventy copies of the samples were tested positive. In general, PVY was the predominant virus, followed by PVS. 33 samples were infected by multiple viruses, among which the combination of PVY+PLRV was the highest, followed by PVS+PVY. PVS was commonly found in the in vitro plantlets, while PVY was quite frequent in microtuber and seed potatoes. |
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