李发玲,李景富,康立功,张贺,许向阳*.与番茄枯萎病抗病基因I-1连锁的AFLP和SSR分子标记[J].植物保护,2011,37(1):37-40. |
与番茄枯萎病抗病基因I-1连锁的AFLP和SSR分子标记 |
AFLP and SSR molecular markers linked to Fusarium wilt resistance gene I-1 of tomato |
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DOI: |
中文关键词: 番茄 枯萎病 AFLP标记 SSR标记 |
英文关键词:tomato Fusarium wilt AFLP molecular marker SSR molecular marker |
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中文摘要: |
本研究以番茄枯萎病抗病品种‘05045’与感病品种‘051451’为亲本配制杂交组合。用870对AFLP引物及319对SSR引物对348个F2代分离群体进行连锁分析,得到4个与番茄枯萎病抗病基因I-1连锁的AFLP标记和2个SSR标记,分别是E41M60 D、E41M62 C、E86M36 B、E32M44 E和SSR108、SSR276,与抗病基因I-1的连锁遗传距离分别为4.7、5.3、8.9、11.5cM和6.1、9.3cM。 |
英文摘要: |
In this study, a cross was made between the resistant tomato variety ‘05045’ and the variety‘ 051451’ susceptible to Fusarium wilt. AFLP and SSR analyses of 348 F2 progenies were performed using 870 AFLP primer combinations and 319 SSR primer combinations. Four AFLP and 2 SSR markers linked to the Fusarium wilt resistance gene were identified. Four AFLP markers E41M60 D, E41M62 C, E86M36 B and E32M44 E, and two SSR markers SSR108 and SSR276 were 4.7、5.3、8.9、11.5、6.1cM and 9.3cM from the wilt resistance gene I 〖STBX〗1〖STBZ〗, respectively. |
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