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赵培宝1,2,任爱芝2,胡明江2,张秀省2,陈蕾蕾1,宋晓妍1,石梅1.农杆菌介导的拟康氏木霉遗传转化及T-DNA插入突变体的筛选[J].植物保护,2010,36(6):74-76.

农杆菌介导的拟康氏木霉遗传转化及T-DNA插入突变体的筛选

Agrobacterium tumefaciens mediated transformation of Trichoderma pseudokoningii SMF2 and isolation of the T DNA insertion mutant

DOI：

中文关键词:

英文关键词:Trichoderma pseudokoningii SMF2 ATMT genetic transformation

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作者	单位
赵培宝1,2,任爱芝2,胡明江2,张秀省2,陈蕾蕾1,宋晓妍1,石梅1

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中文摘要:

英文摘要:

By using Agrobacterium tumefaciens mediated transformation, we successfully transformed Trichoderma pseudokoningii SMF2 and obtained T DNA insertion mutants. Under the optimal conditions (106spores/mL, A. tumefaciens A 600=0.15-0.20, 250 μmol/mL acetosyringone and 36 h co cultivation), the transformation efficiency reached 60-120 transformants per 106spores. More than 1 000 transformants were obtained. Most of them were quite stable after five rounds of successive cultures, and some mutants were abnormal in morphology or spore formation. PCR amplification showed that the T DNA had been integrated into the genome, and was stable through mitotic cell division.

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