| 窦剑1，田艳丽2，陈玉林1，汤诗杰1，胡白石2*.朗伍德王莲腐霉叶腐病及病原鉴定[J].植物保护,2010,36(5):183-185. |
| 朗伍德王莲腐霉叶腐病及病原鉴定 |
| Pythium leaf rot of Victoria ‘Longwood Hybrid’ and identification of the pathogen |
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| DOI： |
| 中文关键词: |
| 英文关键词:Victoria ‘Longwood Hybrid’ leaf rot Pythium helicoides identification of the pathogen |
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| 中文摘要: |
| 2009年，南京中山植物园王莲育苗池中的朗伍德王莲（Victoria ‘Longwood Hybrid’）发生了叶片腐烂病。按照柯赫氏法则对其进行了病原物的分离、纯化和致病性测定，并对菌株进行了形态学和分子生物学鉴定。从发病部位稳定分离到形态一致的低等真菌；致病性测定结果表明，回接后发病株产生与自然侵染相同的症状，并重新分离到相同菌株。通过病原菌形态特征和核糖体rDNA ITS序列分析，病株分离物被鉴定为旋柄腐霉（Pythium helicoides）。这是国内外首次报道在王莲上分离出旋柄腐霉并证明其为王莲的致病菌。该病害被定名为王莲腐霉叶腐病。 |
| 英文摘要: |
| A leaf rot disease of Victoria ‘Longwood Hybrid’ in Nanjing of Jiangsu Province was found. The pathogen was isolated and identified by the Koch’s rule as Pythium helicoids, based on morphological observation and ITS sequence analysis. After inoculation, the isolate could infect Victoria ‘Longwood Hybrid’ and cause the disease. This is the first report that P. helicoids could infect Victoria ‘Longwood Hybrid’. |
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