| 巩洁，陈亮，陈立，韩文霞，陈五岭*.紫外线、He-Ne激光对葡萄白腐病菌拮抗菌PT2的复合诱变效应[J].植物保护,2010,36(5):105-109. |
| 紫外线、He-Ne激光对葡萄白腐病菌拮抗菌PT2的复合诱变效应 |
| Mutagenetic effects of UV and He-Ne laser on the strain PT2 with antagonistic ability against Coniella diplodiella |
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| DOI： |
| 中文关键词: |
| 英文关键词:UV He-Ne laser Bacillus subtilis Coniella diplodiella compound mutation |
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| 中文摘要: |
| 采用紫外线、He-Ne激光及两者复合诱变的方法，分别处理对葡萄白腐病有拮抗作用的枯草芽胞杆菌PT2,以提高其抑菌效果。结果表明，紫外线和He-Ne激光单独作用，所筛菌株UV10与HN14抑菌效果提高率分别为15.0%与21.7%。而经过对复合诱变条件确定，在紫外线照射90 s后，再用15 mW的He-Ne激光辐照10 min，筛选出最佳正变菌株F15，抑菌效果提高30.9%，明显高于单因素诱变，经25代传代试验证明其拮抗能力可以进行稳定遗传。 |
| 英文摘要: |
| The strain PT2 Bacillus subtilis, which has an antagonistic ability to Coniella diplodiella Petrak & Syd. was irradiated respectively by UV and He-Ne laser, and the mutant strains UV10 and HN14 were obtained. Both of them increased the antagonistic ability by 15.0% and 21.7% compared with PT2. The results indicated that the coordinative effect of UV and He-Ne laser was better than that by using them individually. The cells were treated firstly by UV for 90 seconds and then by He-Ne laser for 10 minutes in 15mW. The best positive mutation strain F15 was obtained, whose antagonistic ability was 30.9% higher than PT2’s. By cultivating it from 1st to 25th generation, its antagonistic ability proved to be stable in heredity. |
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