| 唐前君1，孙书娥2,3，刘勇2,3，谭新球2，肖启明1，谢丙炎4，张德咏2,3*.番茄斑萎病毒核衣壳蛋白基因的克隆与分析[J].植物保护,2010,36(5):65-69. |
| 番茄斑萎病毒核衣壳蛋白基因的克隆与分析 |
| Cloning and sequence analysis of the nucleoprotein genes of Tomato spotted wilt virus isolates from Yunnan |
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| DOI： |
| 中文关键词: |
| 英文关键词:Tomato spotted wilt virus nucleoprotein clone |
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| 中文摘要: |
| 从云南表现褪绿黄化症状的番茄上分离到番茄斑萎病毒（Tomato spotted wilt virus）YN-1株系，利用RT-PCR方法克隆了该株系的核衣壳蛋白基因，并测定了该基因的序列，全长为777 bp，编码258个氨基酸。对该基因的氨基酸序列分析结果表明，YN-1 N基因与韩国3个株系的氨基酸序列相似性均高达97%。 |
| 英文摘要: |
| Tomato spotted wilt virus (TSWV) was detected via RT-PCR from tomato with mottle symptom collected from Yunnan. The N gene, encoding nucleoprotein, was cloned and sequenced. Sequence and analysis results indicated that the N gene was 777 bp in length and encoded 258 amino acids. Sequence analyses showed N gene shared 99% and 97% nucleotide identities with the isolates from South Korea and Taiwan, respectively. In amino acid levels, the sequence identity was 99% and 98%, respectively. |
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