| 卢全有.桑树黄化型萎缩病植原体延伸因子基因的克隆及序列分析*[J].植物保护,2010,36(5):43-46. |
| 桑树黄化型萎缩病植原体延伸因子基因的克隆及序列分析* |
| Cloning and sequence analysis of tuf gene for elongation factor Tu of phytoplasma associated with mulberry yellow dwarf disease |
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| DOI： |
| 中文关键词: |
| 英文关键词:mulberry yellow dwarf phytoplasma elongation factor Tu phylogenetic tree |
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| 中文摘要: |
| 利用已报道的引物对fTufAy/rTufAy，采用PCR技术对桑树黄化型萎缩病植原体延伸因子（EF-Tu）tuf 基因片段进行了扩增、测序及序列分析。结果表明，从表现黄化型萎缩病症状的桑树样品中扩增得到预期大小的目的片段。经核苷酸序列测定，扩增得到的延伸因子基因片段为946 bp（GenBank登录号为：GQ268317）。对桑树黄化型萎缩病植原体及16Sr I组中的各亚组代表植原体的延伸因子tuf基因的相似性比较结果表明，桑树黄化型萎缩病植原体与16Sr I组中的MD、PRIVA亲缘关系最近，核苷酸的相似性为99.9%。该序列与已知的16Sr I各亚组代表植原体构建的进化树表明，桑黄化型萎缩病植原体与tuf I B亚组聚类为一个亚组，归为翠菊黄化植原体组(Candidatus Phytoplasma asteris)，即16Sr I组tufI-B亚组，该结果从亚组水平上进一步确定了桑树黄化型萎缩病植原体的分类地位。 |
| 英文摘要: |
| The fragment of tuf gene for elongation factor Tu (EF-Tu)of phytoplasma associated with mulberry yellow dwarf disease (MYD) was amplified by polymerase chain reactions (PCR) using primer pairs fTufAy/ rTufAy, and the amplified fragment was cloned, sequenced and analyzed. The results showed that a 946 bp (GenBank accession No. GQ268317) fragment was amplified from the total DNA samples extracted from the infected mulberry. A phylogenetic tree was constructed by comparing tuf gene sequence of MYD phytoplasma with those of other representative phytoplasma strains in 16Sr I group from GenBank and showed that MYD phytoplasma was clustered into tuf I-B subgroup in 16Sr I group. |
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