| 许修宏.小核盘菌菌丝生长和乙二酸合成影响因素研究[J].植物保护,2009,35(3):57-59. |
| 小核盘菌菌丝生长和乙二酸合成影响因素研究 |
| Factors influencing mycelial growth and oxalic acid production by Sclerotinia minor |
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| DOI： |
| 中文关键词: 小核盘菌 生长 乙二酸 |
| 英文关键词: |
| 基金项目:黑龙江省科技攻关项目，哈尔滨市科技攻关计划项目，东北农业大学创新团队发展计划项目? |
| 许修宏 |
| 东北农业大学,哈尔滨150030 |
| 摘要点击次数: 455 |
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| 中文摘要: |
| 小核盘菌菌丝在V8溶液培养基和酵母浸膏溶液培养基中生长显著优于其他供试培养基，菌丝干重分别为6.666 mg/mL和6.632 mg/mL。在葡萄糖土豆浸汁培养基中产生的乙二酸量显著高于其他培养基，为1.981 mg/mL。在蔗糖液培养基中，培养7 d菌丝干重达到最大，为4.455 mg/mL。培养6 d乙二酸产生量达到最大，为0.966 mg/mL。培养基初始pH4.5最适合菌丝生长，干重为4.678 mg/mL。乙二酸含量随初始pH增加而增加，pH7.0的培养基中乙二酸含量为1.835 mg/mL。氮源含量增加可以促进乙二酸的合成，当蔗糖与大豆水解蛋白之比为25 g/10 g时，乙二酸含量为1.897 mg/mL。添加琥珀酸钠乙二酸含量显著增加，为3.741 mg/mL。 |
| 英文摘要: |
| The mycelia of Sclerotinia minor grew better in V8 juice medium and yeast extract than in other media tested, with the mycelial dry weight as 6.666 mg/mL and 6.632 mg/mL, respectively. S. minor produced significantly more oxalic acid (1.981 mg/mL) in the potato dexterose broth (PDB) medium than in other media. In the sucrose solution medium, the mycelial dry weight reached the peak (4.455 mg/mL) 7 d after inoculation, and the highest oxalic acid was observed (0.966 mg/mL) 6 d after inoculation. The optimum initial pH value for growth of the mycelium was pH4.5 with the dry weight of 4.678 mg/mL. Higher initial pH value favored the oxalic acid accumulation, which reached 1.835 mg/mL at pH 7.0. Higher nitrogen content of soluble soybean protein promoted oxalic acid production, which reached 1.897 mg/mL at the C/N ratio of 25 g/10 g. Medium supplemented with sodium succinate produced significantly more oxalic acid (3.741 mg/mL). |
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