| 叶青静,杨悦俭,王荣青,李志邈,阮美颖,周国治,姚祝平.应用PCR方法检测烟粉虱传双生病毒[J].植物保护,2008,34(6):25-28. |
| 应用PCR方法检测烟粉虱传双生病毒 |
| Detection of whitefly-transmitted geminiviruses by reverse transcription-polymerase chain reaction |
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| DOI： |
| 中文关键词: 番茄黄化曲叶病毒 PCR 检测 |
| 英文关键词:Tomato yellow leaf curl virus PCR detection |
| 基金项目: |
| 叶青静 杨悦俭 王荣青 李志邈 阮美颖 周国治 姚祝平 |
| 浙江省农业科学院蔬菜研究所，杭州310021 |
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| 中文摘要: |
| 番茄黄化曲叶病毒属于双生病毒，根据其外壳蛋白基因保守序列设计简并引物，对杭州和海宁地区番茄上疑似番茄黄化曲叶病毒进行PCR检测，获得产物克隆到pMD18-T载体中，经测序和序列分析，表明杭州和海宁地区发生的病毒是番茄黄化曲叶病毒，并且两地区病毒核苷酸序列同源性达97.5%。 |
| 英文摘要: |
| Tomato yellow leaf curl virus (TYLCV) is a geminivirus transmitted by the whitefly Bemisia tabaci. A pair of degenerate primers was designed according to the conserved sequence of coat protein genes of TYLCV.The tomato samples showing TYLCD-like symptoms were collected from Hangzhou and Haining and detected by PCR for virus presence.PCR products (about 361bp) were then cloned into the vector pMD18-T.Sequence analysis indicated that the nucleotide sequences of the amplified products from Hangzhou and Haining have 97.5% identity, and they share the highest sequence identity with TYLCV, indicating these samples were infected by TYLCV. |
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