| 张秋娥;曹克强;胡同乐;王树桐;杨军玉;王培培.河北省小麦全蚀病菌变种类型鉴定[J].植物保护,2008,34(4):18-21. |
| 河北省小麦全蚀病菌变种类型鉴定 |
| Identification of the varieties of Gaeumannomyces graminis causing wheat take-all in Hebei |
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| DOI： |
| 中文关键词: 小麦全蚀病 病原 禾顶囊壳小麦变种 |
| 英文关键词:wheat take-all pathogen Gaeumannomyces graminis var.tritici |
| 基金项目: |
| 张秋娥;曹克强;胡同乐;王树桐;杨军玉;王培培 |
| 河北农业大学植物保护学院,保定 071001 |
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| 中文摘要: |
| 2007年从河北省的保定、石家庄、邢台小麦主产区采集小麦全蚀病株,共分离得到62个菌株,对其所属的变种类型进行了形态学和分子生物学鉴定。根据形态、培养性状、生理特性以及在小麦、高粱、水稻、玉米、燕麦等禾本科作物上的致病性,初步认定测定的所有菌株均为禾顶囊壳小麦变种(Gaeumannomyces graminisvar.tritici)。进一步采用4个变种的特异性引物进行PCR扩增,在所有菌株中扩增出870bp的条带,该片段为禾顶囊壳小麦变种特异性片段,证实所测菌株均为禾顶囊壳小麦变种。 |
| 英文摘要: |
| Diseased wheat plants caused by the take-all fungi were collected from Baoding,Shijiazhuang and Xingtai of Hebei Province in 2007.The 62 strains isolated from wheat samples were preliminarily identified as Gaeumannomyces graminis var.tritici based on their morphology,physiology and pathogenicity to wheat,sorghum,rice,maize and oat.By using 4 variety-specific primers,a 870bp band was amplified in all strains.The result further confirmed that all of the strains belong to G.graminis var.tritici. |
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