| 胡海燕1;刘爱英1;邹晓1;梁宗琦1;罗力1;申吉秀2;金荣煜2;朴海雄2.虫生真菌蝉拟青霉及其角变株的比较研究[J].植物保护,2008,34(2):21-25. |
| 虫生真菌蝉拟青霉及其角变株的比较研究 |
| A comparative study on the entomogenous fungus Paecilomyces cicadae and its sectors |
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| DOI： |
| 中文关键词: 蝉拟青霉 角变 多糖 酶 致病性 |
| 英文关键词:Paecilomyces cicadae sector exopolysacharides enzyme pathogenicity |
| 基金项目: |
| 胡海燕1;刘爱英1;邹晓1;梁宗琦1;罗力1;申吉秀2;金荣煜2;朴海雄2 |
| 1.贵州大学真菌资源研究所 贵阳550025;2.韩国延世大学 首尔120-749 |
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| 中文摘要: |
| 蝉拟青霉GZUIFR-JZD3菌株在沙氏培养基上可产生孢子型(JZD3-C)和菌丝型(JZD3-M)两类不同的角变。为了进一步研究其差异,笔者比较了两个角变株与原始菌株(JZD3)在不同培养基上的生长及产孢量。研究了其对甘蓝蚜的致病性并测定了它们的胞外多糖、蛋白酶、蛋白质、SOD和POD的含量。结果表明:3个菌株形态差异较大,在多数培养基上,JZD3生长最快,产孢量最大;胞外多糖、SOD、POD含量以及对蚜虫致病率也是JZD3菌株最佳;而蛋白酶和蛋白质含量则分别是JZD3-C和JZD3-M较高。 |
| 英文摘要: |
| Two sectors,spore-type(JZD3-C) and hypha-type(JZD3-M) were isolated from Paecilomyces cicadae on the Sabouraod medium.The colony characters and conidiation between the two sector isolates and the original strain(JZD3) were compared on different media.The contents of exopolysacharides,proteinase,protein,SOD,POD and the pathogenicity to Brevicoryne brassicae were tested. The results showed that the growth of the isolate JZD3 was the fastest and the conidiation was the largest on most media.As far as the contents of exopolysacharides,SOD,POD and the pathogenicity to B.brassicae,JZD3 was also the best;however,the isolates JZD3-C and JZD3-M had the most proteinase and proteins,respectively. |
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