| 李灿1,2;李子忠1;郑兴旺3;周波3;曹宇3.高浓度CO2气调胁迫对药材甲老熟幼虫磷酸酯酶活力的影响[J].植物保护,2008,34(1):123-126. |
| 高浓度CO2气调胁迫对药材甲老熟幼虫磷酸酯酶活力的影响 |
| Effects of carbon dioxide-enriched atmosphere on the activity of phosphatases from mature larvae of Stegobium paniceum |
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| DOI： |
| 中文关键词: 药材甲 高浓度CO2气调 磷酸酯酶 比活力 |
| 英文关键词:Stegobium paniceum(L.) CO2-enriched atmosphere phosphatases specific activity |
| 基金项目: |
| 李灿1 2;李子忠1;郑兴旺3;周波3;曹宇3 |
| 1.贵州大学昆虫研究所贵州山地农业病虫害重点实验室 贵阳550025;3.贵州大学农学院 贵阳550025;2.贵阳学院生物与环境工程系 贵阳550005 |
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| 中文摘要: |
| 用高浓度CO2气体胁迫处理药材甲老熟幼虫及体外磷酸酯酶,通过终点法测定酶活性变化,分析气调胁迫与磷酸酯酶活性变化之间的关系。结果显示,高浓度CO2密闭处理药材甲老熟幼虫6 h和12 h,ACP比活力分别升高6.41%和18.96%,ALP活力分别降低了7.69%和7.91%。ACP经CO2体外处理30、60、120 min,比活力分别增强92.77%、127.43%和67.91%。可见,高浓度CO2气调胁迫处理对药材甲老熟幼虫ACP和ALP活力均有一定影响。 |
| 英文摘要: |
| Under stress of CO2-enriched atmosphere(CA),the activities of phosphatases from the mature larvae of Stegobium paniceum(L.) were determined by using endpoint method.The results showed that if the insects were exposed to CA for 6h or 12 h,the specific activity of ACP increased by 6.41% and 18.96%,respectively,but the activity of ALP declined by 7.69% and 7.91%,respectively.When ACP was treated with CO2 in vitro for 30 min,60 min and 120 min at 37 ℃,its specific activity increased by 92.77%,127.43% and 67.91%,respectively.Therefore,CA might contribute to the change of phosphatases.These data can be used in the research and analysis of the mechanisms for pest control with CA and resistance. |
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