| 秦碧霞1;蔡健和1;刘志明1;魏源文2;谢玲1;朱桂宁1.侵染葫芦的黄瓜绿斑驳花叶病毒广西分离物分子鉴定[J].植物保护,2008,34(1):116-118. |
| 侵染葫芦的黄瓜绿斑驳花叶病毒广西分离物分子鉴定 |
| Molecular identification of an isolate of Cucumber green mottle mosaic virus infecting bottle gourd in Guangxi |
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| DOI： |
| 中文关键词: 黄瓜绿斑驳花叶病毒 外壳蛋白基因 序列分析 鉴定 |
| 英文关键词:Cucumber green mottle mosaic virus coat protein gene sequence analysis identification |
| 基金项目: |
| 秦碧霞1;蔡健和1;刘志明1;魏源文2;谢玲1;朱桂宁1 |
| 1.广西农业科学院植物保护研究所 南宁530007;2.广西作物遗传改良生物技术重点开放实验室 南宁530007 |
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| 中文摘要: |
| 从广西南宁市郊温室大棚中的葫芦[Lagenaria siceraria(Molina)Stand.]上采集到一个表现脉绿、花叶症状的病毒样品,ELISA检测表明,该样品与黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaic virus,CGMMV)有密切的血清学关系,利用RT-PCR方法从样品中扩增获得约500bp的DNA片段,序列分析表明,该片段是CGMMV的外壳蛋白基因,暂将该病毒分离物定名为GX-BG。外壳蛋白基因核苷酸序列系统进化树分析表明,已报道的CGMMV主要分为3大群体,GX-BG与中国辽宁分离物(CGMMV-LN)分别属于不同的群体。 |
| 英文摘要: |
| A virus isolate from bottle gourd(GX-BG) with green veins and mosaic symptoms was collected in greenhouses in the suburb of Nanning.ELISA tests indicated that GX-BG had close serological relationships with Cucumber green mottle mosaic virus(CGMMV).By using specific primers designed based on the coat protein gene of CGMMV,a 500-bp segment was obtained by RT-PCR.A BLAST search confirmed that it was an isolate of CGMMV.The previously reported CGMMVs could be classified as three main groups according to phylogenetic analysis based on the coat protein gene nucleotide sequences of CGMMV.GX-BG and CGMMV-LN belonged to different groups. |
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