| 梁巧兰;魏列新;徐秉良;师桂英;谢忠魁.观赏百合病毒病原形态及内含体研究[J].植物保护,2008,34(1):95-98. |
| 观赏百合病毒病原形态及内含体研究 |
| Morphology of the viral pathogens infecting ornamental lily and their inclusion bodies |
| |
| DOI： |
| 中文关键词: 观赏百合 病毒病原 形态 内含体 |
| 英文关键词:ornamental lily viral pathogens shape inclusion bodies |
| 基金项目: |
| 梁巧兰;魏列新;徐秉良;师桂英;谢忠魁 |
| 甘肃农业大学草业学院 兰州730070; |
| 摘要点击次数: 647 |
| 全文下载次数: 835 |
| 中文摘要: |
| 通过带病组织汁液和带病组织超薄切片负染电镜技术对侵染观赏百合的病毒粒子和内含体进行观察,证明在表现不同症状的5个品种中存在球状、短线状、长线状、短杆状4种病毒粒子和晶体状、风轮状、束状、卷筒状、正六边形5种内含体,通过ELISA检测及病毒粒子形态、大小及内含体形态分析,确定球状病毒粒子(直径29.5 nm)为黄瓜花叶病毒,短线状病毒粒子(593.29～639.97 nm×12.15～14.12 nm)为百合无症病毒,长线状病毒粒子(757.58～846.25 nm×12.12～12.75 nm)为马铃薯Y病毒,短杆状病毒粒子(500～807.69 nm×153.85～192.31 nm)为烟草脆裂病毒。 |
| 英文摘要: |
| Four kinds of virus particles with spherical,fibrous(long,short) and short-rod shapes were found and the inclusion bodies of crystalloid,hexagon,bundle,scroll and pinwheel were observed in the cytoplasm of mesophyll cells by electron microscope.Enzyme-linked immumnosorbent assay(ELISA) indicated that the spherical and short fibrous viruses were cucumber mosaic virus(29.5 nm) and lily symptomless virus(593.29~639.97 nm×12.15～14.12 nm),while the long fibrous and short rod-shaped viruses were preliminarily identified as Potyvirus(757.58~846.25 nm×12.12~12.75 nm) and tobacco rattle virus(500～807.69 nm×153.85~192.31 nm). |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
