宋丽雯;杨顺义;张新虎;沈慧敏.顶孢霉Ahy1营养条件的初步研究[J].植物保护,2007,33(6):67-70. |
顶孢霉Ahy1营养条件的初步研究 |
A preliminary study on the nutritional conditions of Acremonium hansfordii |
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DOI: |
中文关键词: 顶孢霉 营养条件 碳源 氮源 |
英文关键词:Acremonium hansfordii nutritional conditions carbon sources nitrogen sources |
基金项目: |
宋丽雯;杨顺义;张新虎;沈慧敏 |
甘肃农业大学草业学院 兰州730070 |
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中文摘要: |
顶孢霉(Acremonium hansfordii)在PDA、淀粉琼脂、查彼氏、萨氏培养基和萨氏蛋黄培养基上培养,萨氏培养基和萨氏蛋黄培养基有利于菌落生长和产孢,菌落直径和产孢量显著高于其他处理,分别为7.77 cm和7.07 cm,19.02×103、25.87×103个孢子;葡萄糖、果糖、蔗糖、淀粉、甘露醇、肌醇、甘油、麦芽糖8种碳源中葡萄糖、蔗糖有利于菌落的生长和产孢,菌落直径分别为7.73 cm和7.60 cm,产孢量分别为25.96×103、25.79×103个,显著高于其他处理;蛋白胨、硝酸钠、硝酸钾、氯化铵、硝酸铵、尿素6种氮源中,蛋白胨有利于菌落的生长和产孢,菌落直径为7.60 cm,产孢量为25.91×103个,显著高于其他处理。 |
英文摘要: |
Eight carbon sources(glucose,fructose,saccharose,starch,mannite,inosite,glycerine and maltose) and six nitrogen sources(peptone,sodium nitrate,potassium nitrate,ammonium chloride,ammonium nitrate and urea) were examined for their effect on the growth and sporulation of Acremonium hansfordii.It was found that glucose,saccharose and peptone were the best.The five cultures(PDA,Sarch-Agar,Czapek,SDAY,SEA) were screened.As a result,SEA and SDAY were the optimum culture media. |
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