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李亚利1;贾迎春2;蒲崇建2;杨成德1;陈秀蓉1.采用16S rDNA鉴定甜瓜细菌性叶斑病菌[J].植物保护,2007,33(3):65-68.
采用16S rDNA鉴定甜瓜细菌性叶斑病菌
Identification of the pathogen of bacterial spot of cucurbits leaves based on 16S rDNA sequences
  
DOI:
中文关键词:  细菌性叶斑病  致病性测定  16S rDNA鉴定
英文关键词:bacterial leave spot  pathogenicity  identification based on 16S rDNA
基金项目:
李亚利1;贾迎春2;蒲崇建2;杨成德1;陈秀蓉1
1.甘肃农业大学草业学院 兰州730070;2.甘肃省植保植检总站 兰州730030
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中文摘要:
      从甘肃河西、新疆阿勒泰甜瓜上分离获得的2株致病细菌,通过16S rDNA序列测定以及序列同源性比较,结合病原菌落培养性状、菌体形态观察和革兰氏染色反应等,初步确定当地甜瓜细菌性叶斑病菌为丁香假单胞杆菌[Pseudomonas syringae pv.lachrymans (Smith et Bryan) Young et al.]
英文摘要:
      Two strains of pathogenic bacteria were isolated in Hexi in Gansu and Aletai in Xinjiang.The strains were examined using 16S rDNA gene sequences.The results showed that the pathogens might be Pseudomonas syringae pv.lachrymans(Smith et Bryan) Young et al.,based on their culture patterns,morphological characteristics and staining reactions.
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