晏卫红1;黄思良1,3;王永娇2;胡春锦1,3.中国薯蓣干腐病的病原鉴定[J].植物保护,2007,33(3):53-57. |
中国薯蓣干腐病的病原鉴定 |
Identification of the causal agent of dry rot disease of yam in China |
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DOI: |
中文关键词: 薯蓣 干腐病 Penicilliumsclerotigenum 生物学特性 |
英文关键词:yam dry rot disease Penicillium sclerotigenum biological characteristics |
基金项目: |
晏卫红1;黄思良1 3;王永娇2;胡春锦1 3 |
1.广西农业科学院植物保护研究所 南宁 530007;2.长江大学农学院 荆州 434025;3.广西农业科学院植物保护研究所 南宁530005 |
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中文摘要: |
在薯蓣发病块茎组织中分离到真菌菌株HSQM2005,经柯赫氏法则验证该菌为薯蓣致病菌。对菌株HSQM2005核糖体DNA内转录间隔区(internal transcribed spacer,ITS)进行序列分析,并结合其形态特征、培养性状将该菌鉴定为Penicillium sclerotigenum Yamamoto。该菌生长温度范围10~31℃,最适温度22~28℃;在pH 2~12均能生长,最适pH为5~8。 |
英文摘要: |
A fungal strain HSQM2005 was isolated from the diseased tuber tissue of yam in Guangxi,China.The pathogenicity of the strain was confirmed by inoculation and re-isolation tests on the tubers of yam.The ITS sequence of rDNA from the strain was analyzed.The pathogenic strain HSQM2005 showed 100% homology in ITS sequence with that of Penicillium sclerotigenum Yamamoto retrieved from GenBank.The growth temperature of the strains varied from 10 ℃ to 31 ℃,and 22-28 ℃ was the most suitable temperatures for the growth of the pathogen.The pathogen could grow on media with pH values ranging from 2 to 12.The media with pH 5-8 were most suitable for its growth.This is the first record on the occurrence of yam dry rot disease caused by P.sclerotigenum in China.P.sclerotigenum is a new species recorded in China. |
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