| 郑耘1;杨伟东1;陈枝楠1;李明福2;章桂明1;余道坚1;张永江2.烟草环斑病毒DB-RT-Realtime PCR检测方法研究[J].植物保护,2007,33(1):117-120. |
| 烟草环斑病毒DB-RT-Realtime PCR检测方法研究 |
| A study on detection of Tobacco ringspot virus by DB-RT-Realtime PCR |
| |
| DOI： |
| 中文关键词: 烟草环斑病毒 反转录PCR 检测 |
| 英文关键词:Tobacco ringspot virus reverse-transcription PCR detection |
| 基金项目: |
| 郑耘1;杨伟东1;陈枝楠1;李明福2;章桂明1;余道坚1;张永江2 |
| 1.深圳出入境检验检疫局 深圳518001;2.中国检验检疫科学院动植物检疫研究所 北京100025 |
| 摘要点击次数: 944 |
| 全文下载次数: 470 |
| 中文摘要: |
| 本研究首次应用直接结合反转录实时荧光PCR技术(direct binding reverse transcription realtime PCR,DB-RT-Re-altime PCR)检测烟草环斑病毒,由于综合运用了PCR管吸附病毒外壳蛋白、病毒核酸分子杂交、高灵敏度实时荧光PCR技术的优点,从而使病毒检测在特异性、灵敏度、稳定性等技术指标上比传统的DAS-ELISA方法都有所提高,解决了烟草环斑病毒检测工作中由于隐症、干扰物质存在而影响检测结果的问题,为病毒检测提供了一个快速、简便有效的检测方法。 |
| 英文摘要: |
| Direct binding reverse transcription realtime PCR technique was used for detecting Tobacco ringspot virus.For the advantages of combining capsid proteinabsorption by PCR tubes and molecular hybridization of virus nucleic acids with high sensitivity of Real-Time PCR,this technique is better in detection specificity,sensitivity and stability than the traditional DAS-ELISA method.It also resolved the problems such as symptom-masking and interfering materials in detecting Tobacco ringspot virus. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
