| 杨小军1,2;倪汉文2;杨立军1;王少南1;喻大昭1.采用孢子萌发法测定化合物对黄瓜白粉病菌的生物活性[J].植物保护,2007,33(1):75-77. |
| 采用孢子萌发法测定化合物对黄瓜白粉病菌的生物活性 |
| A novel bioassay method—determining the bioactivity of chemical compounds to cucumber powdery mildew by spore germination |
| |
| DOI： |
| 中文关键词: 孢子萌发法 白粉病菌 生物活性 |
| 英文关键词:spore germination method powdery mildew bioactivity |
| 基金项目: |
| 杨小军1 2;倪汉文2;杨立军1;王少南1;喻大昭1 |
| 1.湖北省农业科学院植保土肥研究所 武汉 430064;2.中国农业大学农学与生物技术学院 北京100094 |
| 摘要点击次数: 769 |
| 全文下载次数: 675 |
| 中文摘要: |
| 接种黄瓜白粉菌后16 h的黄瓜叶片用透明胶带在子叶表面粘孢子取样的平均发芽率与用台盼蓝染色后在光学显微镜下调查的发芽率分别为83.7%和85.0%,t测验无显著差异,表明透明胶带取样的发芽率可以代表黄瓜叶片上白粉病菌孢子的发芽率。采用孢子萌发法和叶碟法分别测定了醚菌酯、植物源活性组分大黄素甲醚(P3D)对8个黄瓜白粉菌菌株的EC50。统计结果表明:采用两种方法测定醚菌酯P、3D对黄瓜白粉菌的EC50的相关系数的平方值分别为0.880、.99,表明两种方法的测定结果有很强的相关性;采用该孢子萌发法可评价化合物对植物专性寄生病菌如白粉菌孢子萌发的生物活性。 |
| 英文摘要: |
| The reliability of a novel bioassay method that was designed to determine the bioactivity of chemical compounds to powdery mildew based on spore germination inhibition was studied.The results showed that sampling spore germination by transparent tapes was simple and reliable.There was a significant correlation between the results by spore germination method and that by leaf disc assay no matter whether synthetic fungicide kresoxim-methyl or biofungicide P3D was used on cucumber powdery mildew.The results indicated that the spore germination method could replace the leaf disc method in fungicide bioassay against this disease. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
