| 罗青;巫鹏举;李晓莺;贝盏临;曲玲;曹有龙.转雪花莲凝集素酶基因枸杞对蚜虫的抑制作用[J].植物保护,2007,33(1):65-67. |
| 转雪花莲凝集素酶基因枸杞对蚜虫的抑制作用 |
| Inhibition effects of the transgenic Lycium plants expressing snowdrop lectin on development of aphid population |
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| DOI： |
| 中文关键词: 枸杞 雪花莲外源凝集素酶基因 转化 蚜虫 抑制 |
| 英文关键词:Lycium barbarum L. gna gene transformation aphid inhibition |
| 基金项目: |
| 罗青;巫鹏举;李晓莺;贝盏临;曲玲;曹有龙 |
| 宁夏枸杞发展工程技术研究中心 银川750002 |
| 摘要点击次数: 975 |
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| 中文摘要: |
| 利用农杆菌介导法将雪花莲外源凝集素酶基因导入枸杞叶片,获得了完整的转基因植株。对36个经PCR检测为阳性的转基因株系进行了两年抗蚜虫筛选试验,结果表明:蚜口密度抑制率在13.19%～95.4%,平均蚜口密度抑制率为78.59%。 |
| 英文摘要: |
| Agrobacterium-mediated transformation of Lycium was studied to prevent the pest aphid.The gene gna was successfully transferred into Lycium by using leaf dish,and a large number of completely transgenic plants were obtained.Thirty-six transgenic Lycium plants that had been confirmed as being gna-integrated by PCR detection were tested for resistance to aphids in two years.The results showed that the inhibition rate of aphid population was 13.19%-95.4%,78.59% on average. |
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